摘要
目的细胞中端粒（ｔｅｌｏｍｅｒｅ）的长度与细胞寿命的调控密切相关，端粒长度的维持需要端粒酶（ｔｅｌｏｍｅｒａｓｅ）的激活，近年来的研究发现，端粒酶的激活与肿瘤的发生、发展关系密切。本文报道人胃癌和结、直肠癌端粒酶活性。方法采用端粒重复序列扩增法（ｔｅｌｏｍｅｒｉｃｒｅｐｅａｔａｍｐｌｉｆｉｃａｔｉｏｎｐｒｏｔｏｃｏｌ，ＴＲＡＰ）来检测人胃癌（１１例）、结肠癌（９例）和直肠癌（２０例）以及其中３９例相应的癌旁组织中的端粒酶活性。结果在４０例肿瘤组织中，有３６例端粒酶呈阳性（９０％），且与肿瘤发展阶段、恶性程度不相关；而３９例癌旁组织中只有１例阳性。结论端粒酶可能是肿瘤诊断的一个很好的指标。
Objective To study telomerase activity in patients with gastric and colorectal cancer. Methods Telomerase activity in tumor samples, including 9 colonic, 20 rectal and 11 gastric carcinomas, was examined by telomeric repeat amplification protocol(TRAP) assay. Thirty nine samples from tumor adjacent tissues were also assayed. Results Ninety per cent(36 out of 40 tumor samples) exhibited telomerase activity regardless of tumor stage or differentiation. However, telomerase activity in the tumor adjacent tissues could be detected only in 1 of 39 specimens. Conclusion Telomerase may be a good diagnostic biomarker for tumor detection.
出处
《中华肿瘤杂志》
CAS
CSCD
北大核心
1998年第4期261-263,共3页
Chinese Journal of Oncology
基金
国家自然科学基金
关键词
胃肿瘤
结肠肿瘤
直肠肿瘤
端粒酶
肿瘤标记
Stomach neoplasms Colonic neoplasms Rectal neoplasms Telomerase Tumor biomarkers
