摘要
目的：建立HPLC法测定1％盐酸麻黄碱滴鼻剂中盐酸麻黄碱的含量测定方法。方法：采用C18色谱柱（4．6mm×150mm，5μm）；流动相为甲醇-0．01mol／L磷酸二氢钠（60：40）；流速：1ml／min；柱温：室温；检测波长为257nm。结果：盐酸麻黄碱在40—400μg／ml浓度范围内呈良好线性关系（r=0．9999），含量测定平均回收率为101．12％，RSD为1．02％（n=9）。结论：该方法分析操作简便、快速，选择性高并具有一定的灵敏度，适用于医院制剂的质量控制。
Objective： To determine the contents of ephedrine in 1% ephedrine hydrochloride nasal drops by HPLC. Methods： The HPLC analyses was performed on a C18 chromatographic column in ambient temperature, using UV detection at 257 nm. The solution of methanol - 0.01 mol/L sodium biphosphate （ 60： 40 ） was used as a mobile phase, the flow rate of which was 1.0 ml/min. Results ： The linear range of ephedrine was from 40μg/ml to 400 μg/ml （ r = 0. 999 9 ）. The average recovery was 101.12% and RSD was 1.02% （ n = 9）. Conclusion： The method was easy, rapid and accurate for determination of ephedrine and suitable for the quality control for hospital preparations.
出处
《天津药学》
2009年第3期18-19,共2页
Tianjin Pharmacy
