摘要
采用乙醇提取法对展毛翠雀花根部总生物碱进行提取,通过正交试验,对乙醇体积分数、提取温度和提取时间等因素进行优化,确定了提取最佳工艺;同时,对总生物碱粗提物进行抑菌试验。结果表明,展毛翠雀花根部总生物碱乙醇提取的最佳工艺条件为:70%乙醇,60℃条件下提取6 h。经TLC检测,展毛翠雀花根部组织中共含有5种生物碱,醇溶生物碱对金黄色葡萄球菌和黑曲霉有明显的抑菌作用,其最低抑菌浓度均为50 mg/mL。
The total alkaloid was extracted with alcohol from the root of Delphinium grandiflorum, The results shown that the optimum condition was as follows： extracting temperature is at 60 ℃, extracting time is 6 h, and alcohol concentrationis 70%. TLC result showed five kinds of alkaloids were extracted. The total alkaloidl had evidently antibacterial activity only on Staphylococcusaureusand Secret aspergillus, and its minimum concentration was 50 mg/mL.
出处
《大连工业大学学报》
CAS
北大核心
2009年第4期248-250,共3页
Journal of Dalian Polytechnic University
基金
大连科技攻关项目(2004B3SF175)
关键词
展毛翠雀花
生物碱
抑菌试验
提取
Delphinium grandi florum
alkaloid
antibacterial
extraction
