摘要
[目的]为防治鸭疫里默氏杆菌所致鹅群传染病提供理论依据。[方法]对染病鹅群进行流行病学调查,从病鹅中分离鉴定致病菌并通过药敏试验研究其防治方法。[结果]待检菌的纯培养物涂片经革兰氏染色为阴性小杆状,瑞氏染色后呈两端浓染的细小球杆菌。该菌在半固体培养基上沿穿刺线生长,因而被鉴定为鸭疫里默氏杆菌。动物回归试验表明:用病鹅分离菌接种雏鹅、雏鸭后,发病雏鹅和雏鸭的临床、解剖症状与自然病例一致,且能回收到鸭疫里默氏杆菌。该菌对庆大霉素、卡那霉素和壮观霉素敏感,对林可霉素、环丙沙星等中敏,对链霉素、青霉素、磺胺嘧啶和四环素不敏感。[结论]该致病菌为鸭疫里默氏杆菌,其所致鹅群疫病可用庆大霉素、卡那霉素和壮观霉素进行防治。
[ Objective] The aim was to provide theoretical basis for controlling goosery infectious diseases induced by Riemerella anatipestifer. [ Method] The epidemiological investigation was performed on diseased goosery, the pathogen was isolated from diseased goose and identified and its centrol measures were researched through susceptibility test. [ Result ] The pure culture filmpreparation of tested bacterium was negative small rod-shape in gram staining and fine coccobacillus with two dense stained ends in wright staining. The bacterium grew along the puncture line on the semisolid medium, so it was identified as R. anatipestifer. The animal regression experiment indicated that after the goslings and ducklings were inoculated with the bacterial strains isolated from diseased goose, the clinical and anatomical symptoms of diseased goslings and ducklings were consistent with that of natural cases and R. anatipestifer could be isolated from diseased goslings and ducklings. The bacterium was sensitive to gentamicin, kanamycin and spectinomycin, medium sensitive to lincomyciu, eiprofloxacin, and etc. and not sensitive to streptomycin, benzylpenicillin, sulfadiaziue and tetracycline. [ Conclusion ] The pathogen was R. anatipestifer and the goosery blight induced by it could be controlled with gentamicin, kanamycin and spectinomycin.
出处
《安徽农业科学》
CAS
北大核心
2009年第20期9487-9488,共2页
Journal of Anhui Agricultural Sciences
关键词
鹅
鸭疫里默氏杆菌
生化试验
动物回归
药敏试验
Goose
Riemerella anatipestifer
Biochemical test
Animal regression
Susceptibility test
