摘要
本文采用反相高效液相色谱法，可同时分别测定复方苯甲酸酊中水杨酸和苯甲酸的含量。色谱条件：EliteC18色谱柱（4．6mm×220mm）；流动相：甲醇：0．1mol／L，磷酸二氢钠＝60：40（v／v），磷酸调pH值，检测波长265um。方法简便、精确。
A convenient RP-HPLC method used for content determination of compound benzoic acid tincture was studied and established. The traditional neutralization could only determine total acids, but the new HPLC method can determine the content of benzoic acid and salicylic acid respectively without any separating procedure in advance. Chromatography was carried out on a Elite C18 column using methanol: 0.1mol／L NaH2PO4 (60:40, v／v) mobile phase adjusted by H,PO. at a flow rate of 1.0ml／min and the detection wavelength was set at 265nm.
出处
《福建医药杂志》
CAS
1998年第3期95-97,161,共3页
Fujian Medical Journal
