摘要
本文通过测定卵磷脂一纤维素酶—水(PC—Cel一H_2O)体系中纤维素酶三组分(内切葡聚糖酶,外切葡聚糖酶,p-葡萄糖苷酶)的活性及相对应的紫外吸收光谱(包括考察谱峰的位移和强度的消长),建立起一个模型,用于描述PC—Cel一H_2O体系中各组分间相互作用。当磷脂浓度(C_(DC))很小时,其极性头部作用于内切葡聚糖酶,使其构象变化,并通过后者抑制了另两种酶的活性;当增大到一定浓度(即C_(cel)为0.13 mg/mL时,此C_(pc)为0.3mg/mL)时,PC的作用使内切葡聚糖酶完全失活,并开始以非极性尾部作用于外切葡聚糖酶;当大于PC的临界胶束浓度时,外切酶随非极性部位进入胶束或脂双层而失活,内切葡聚糖酶却因PC对其作用变小而部分恢复活性,p-葡萄糖苷酶在后两种情况下均有活性。本研究为纺织行业对纤维素酶各组分活性的有效控制和利用提供了一种方法。
Through determining the activities and relevant UV-spectra (including the movement and change of the peak) of the catalytic position of three elements (EG, CBH and BG) of cellulase in PC-Cel -H2O solution,a model was established to describe the interaction of the elements in these solutions. When Cpc is very small, the PC's polar head affects EG ,and restrains the activities of the other two enzymes by changing EG's structure (when Cpc increases to a certain concentration (Ccel is 0. 13mg/mL,Cpc is 0. 3 mg/mL) ,EG loses the activity completely because of the action of PC,and PC begins to affect CBH by its nonpolar chains; when Cpc is bigger than CMC of PC ,CBH loses its activity with PC's nonpolar chains entering micelle or the lipid bilayer. At the same time,EG regains partly its activity because the action of PC becomes weaker. BG has activity in the latter two situations.
出处
《印染》
北大核心
1998年第5期11-14,共4页
China Dyeing and Finishing
