摘要
利用部分鲤科鱼类中具多态位点的74对微卫星引物对鲮基因组DNA进行筛选扩增，其中11对引物可稳定扩增且有较高的多态性，占总引物数的14．86％，并利用筛选的引物对西江段3个野生鲮群体（深色群体、浅色群体、西江群体）和1个养殖群体进行遗传多样性分析．结果显示：11个引物扩增得到等位基因数为4～23个，大小为100—374bp，平均多态信息含量为0．7498，不同群体的观测杂合度为0．5105～0．6273，期望杂合度为0．7120～0．7656，深色群体、浅色群体、西江群体和养殖群体的Nei氏基因多样度分别为0．7451±0．3884，0．7632±0．3968，0．7081±0．3712，0．7392±0．3852，野生群体与养殖群体相比，杂合度和遗传多样性水平基本一致．运用Genetix软件计算得到4个群体间的基因分化系数为0．0268～0.0703。AMOVA分析表明群体间的变异占总变异的6．96％，群体内个体间的变异占93．04％，固定系数为0．06964，群体间具有一定程度的分化，但分化主要表现在野生群体和养殖群体之间，而野生群体之间的分化不明显．
Seventy four pairs of microsatellite primers developed for other cyprinids were tested for amplification in mud carp, Cirrhina molitoreUa. Eleven primers, 14.86% , which yielded stable and polymorphic products were used to detect the population genetic variation of C. molitorella. Four populations, namely the green-color population, the yellow-color population, were analyzed respectively using the 11 and the fragment sizes ranged from 100 bp to 374 （PIC） was 0. 749 8. The observed heterozygosity of population, the Xijiang population and the cultured microsatellite primers, 4 to 23 alleles were amplified bp, and the mean polymorphic information content the four populations ranged from 0. 510 5 to 0. 627 3 and the expected heterozygosity were 0. 712 0 -0. 765 6. The average gene diversity loci for the four pop- ulations were 0. 745 1±0. 388 4,0. 763 2±0. 396 8,0. 7081 ±0. 371 2,0. 739 2±0. 385 2, respectively. All the indices above indicated marked variations in the four populations and the genetic variation level of the cultured population was consistent with the wild populations, which meant a good genetic condition of the cultured population of C. molitoreUa. The Gst between every two populations calculated with the Genetix software ranged from 0. 026 8 to 0. 070 3, which indicated some differentiation between the populations. The AMOVA analysis also showed that the differentiation was 6.96% variation among populations and 93.04% variation within populations, and the fixation index was 0. 069 64. But the differentiation mainly appeared between the wild population and the cultured population, and little differentiation appeared among wild populations.
出处
《华南农业大学学报》
CAS
CSCD
北大核心
2009年第3期81-85,共5页
Journal of South China Agricultural University
基金
国家自然科学基金(30271022)
国家重点基础研究发展计划项目(2004CB117401)
广东省科技计划项目(2005B20301007
2005A20105001)
国家科技基础条件平台建设项目(2006DKA30470-008)
关键词
鲮
野生群体
养殖群体
PCR扩增
微卫星标记
遗传变异
Cirrhina molitorella
wild population
cultured population
PCR amplificatioin
microsatellite marker
genetic variation
