摘要
目的:分析不同牙周炎症状态下牙龈卟啉单胞菌(Porphyromonas gingivalis,P.gingivalis)rag-1基因mRNA的表达规律。方法:收集150例慢性牙周炎患者的龈下菌斑,PCR法检测P.gingivalis和rag-1基因。对P.gingivalisrag-1基因阳性的菌斑样本,采用RT-PCR法检测rag-1mRNA的表达水平,并比较不同炎症状态下的表达差异。应用SPSS11.0软件包对数据进行配对t检验和秩相关检验。结果:轻度组、中度组和重度组的rag-1基因mRNA的相对表达水平分别为1.19±0.06、0.46±0.05和2.22±0.10(P<0.05)。结论:rag-1基因的mRNA表达水平随着牙周组织破坏程度及炎症状态的不同而改变。
PURPOSE： To analyse the expression of rag-1 of Porphyromonas gingiwdis （P.gingivalis） in different periodontal inflamed conditions. METHODS： 150 subgingival plaque samples were collected from chronic periodontitis patients. The presence of P.gingivalis and rag-1 was determined by PCR. mRNA level of rag-I was assessed in P. gingivalis rag-1 positive samples by RT-PCR. RESULTS： The mRNA level of rag-1 was 1.19±0.06, 0.46±0.05 and 2.22±0.10 （P〈0.05） respectively at the mild, moderate and severe inflammation sites. CONCLUSION： mRNA level of rag-1 may be changed with the different clinical periodontal inflamed condition. Supported by National Natural Science Foundation of China （Grant No.30772427）.
出处
《上海口腔医学》
CAS
CSCD
2009年第3期225-228,共4页
Shanghai Journal of Stomatology
基金
国家自然科学基金(30772427)~~
