摘要
目的:研究人参皂苷Rb1对染铅小鼠血铅水平及行为记忆的影响.方法:以醋酸铅饮水制备染铅小鼠模型,灌胃给予100,50,25mg/kg不同剂量的人参皂苷Rb1,采用Morris水迷宫实验评价人参皂苷Rb1对小鼠学习记忆的影响,并测定小鼠血中铅含量、脑组织中超氧化物歧化酶(SOD)活性和一氧化氮合酶(NOS)活性.结果:染铅可导致小鼠水迷宫实验搜索路程及潜伏期延长;SOD活性降低及NOS活性升高,与空白对照组相比较差异显著(P<0.05或P<0.01).给予人参皂苷Rb1后,染铅小鼠水迷宫实验搜索路程及潜伏期缩短;血铅含量降低;SOD活性升高;NOS活性降低,与染铅模型组相比较差异显著(P<0.05或P<0.01).结论:人参皂苷Rb1能明显降低血铅浓度;通过提高染铅小鼠体内抗氧化系统活力,改善染铅小鼠的学习记忆能力.
AIM： To explore the effects of ginsenoside Rb1 on the blood lead levels and behaviors of lead-exposed mice. METHODS ： Lead-exposed models of mice were established with lead acetate drinking water, and different doses of ginsenoside Rb1 （100, 50, 25 mg/kg） were fed. Morris water maze test was employed to evaluate the effects of ginsenoside Rb1 on the learning and memory functions of the lead-exposed mice. The blood lead content and the activity of superoxide dismutase （SOD） and nitric oxide synthase （NOS） were detected. RESULTS ： Morris water maze test showed that the searching distance and the latent periods were extended in lead-exposure mice. The activity of SOD decreased while the activity of NOS increased compared with those in control group（P 〈0. 05 or P 〈0. 01）. Ginsenoside Rb1 markedly shortened the searching distance and latent periods, decreased the blood lead content, promoted the activity of SOD, and lowered the activity of NOS（P 〈 0. 05 or P 〈 0. 01）. CONCLUSION： The results suggest that ginsenoside Rb1 reduces the blood lead concentration and alleviates the learning and memory obstacles of lead-exposed mice by restoring the vitality of the antioxidant system.
出处
《第四军医大学学报》
北大核心
2009年第13期1239-1241,共3页
Journal of the Fourth Military Medical University
基金
吉林省科技厅发展计划项目(200705405)
