摘要
构建了针对新城疫病毒NDV NA-1株P基因的RNAi质粒;转染质粒36 h后接种NDV,通过对病毒滴度测定、实时荧光定量PCR和细胞病变结果分析,表明其能抑制NDV在CEF中的复制和增殖。本试验为进一步研究NDV复制机理和抗病毒治疗提供了技术基础。
A plasmids of continuous expressing shRNAs targeting the NDV NA-1 Phosphoprotein （P） gene was designed. Virus titration,Real Time RT-PCR,CPE indicated that P-specific siRNA could inhibit virus replication at 36h post-virus infection, In future studies,a combination of siRNAs targeting the NP and L gene may be used as a tool to study NDV replication and antiviral therapy.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2009年第7期841-844,共4页
Chinese Journal of Veterinary Science
基金
国家自然科学基金资助项目(30771606)
