摘要
目的大鼠灌胃黄连-木香药对提取物后,测定盐酸小檗碱在大鼠血浆中的药动学参数,研究药对配伍后对盐酸小檗碱在大鼠血浆中的影响。方法采用高效液相色谱法测定大鼠灌胃后血浆中盐酸小檗碱的含量,色谱柱:DiamonsilC18(150mm×4.6mm,5μm);流动相:乙腈-水(42∶58),每100mL含有0.34g磷酸二氢钾,0.17g十二烷基硫酸钠;流速:1.0mL·min-1;检测波长:349nm,柱温:35℃。结果药对提取物药动学参数:MRT(0-24)(12.470±1.538)h,MRT(0→∞)(29.711±7.176)h,AUC(0→24)(17.797±7.956)mg·L-1·h-1,AUC(0→∞)(92.522±53.784)mg·L-1·h-1,Tmax2h,Cmax(1.360±0.042)mg·L-1。结论黄连、木香药对配伍后,采用DAS2.0软件处理,房室模型拟合分析,药室模型符合一房室模型。
OBJECTIVE The pharmacokinetic parameters of berberine in rat plasma were studied after oral administration to the monomer of drug-pair of Rhizoma Coptidis-Radix Aucklandiae and explored the pharmacokinetics effect on berberine in rat plasma after oral administration to drug-pair. METHODS The HPLC method was used to determine the quantity of berberine in rat plasma. The Diamonsil C18（150 mm×4.6 mm, 5 μm）column was used, the mobile phase consisted of acetonitrile and water （42 ： 58）, including 0.34 g Potassium dihydrogen phosphate and 0.17 g Sodium dodecyl sulfate per 100 mL, the flow rate was 1.0 mL·min^-1, the UV detector was operated at 349 nm and the column temperature was controlled at 35℃. RESULTS Pharmacokinetic parameters of the extract of drug-pair： MRT（0-24）（12.470±1.538）h, MRT（0→∞）（29.711±7.176）h, AUC（0→24） （17.797±7.956） mg·L^-1·h^-1, AUC（0→∞）（92.522±53.784） mg·L^-1·h^-1, Tmax 2 h, Cmax（1.360±0.042） mg·L^-1. CONCLUSION Drug-pair of Rhizoma Coptidis-Radix Aucklandiae is one-compartment model.
出处
《中国现代应用药学》
CAS
CSCD
北大核心
2009年第6期443-446,共4页
Chinese Journal of Modern Applied Pharmacy
关键词
黄连
木香
盐酸小檗碱
药动学
Rhizoma Coptidis
Radix Aucklandiae
berberine
pharmacokinetics
