摘要
用水提醇沉法提取夹竹桃叶多糖(NP),观察其对小鼠S180皮下移植瘤和S180腹水瘤的生长抑制作用,采用免疫组化法检测了移植瘤组织中PCNA、bcl-2、bax蛋白表达的变化,采用药物相互作用指数研究了NP与环磷酰胺联用对S180移植瘤的协同抑制作用.苯酚-硫酸法测定结果表明,提取物中的多糖质量分数为81.14%.NP对小鼠S180移植瘤有显著的抑制作用并且能延长腹水瘤小鼠的寿命,50,100,150mg/kg剂量组的抑瘤率分别为40.00%,50.83%,44.17%,生命延长率分别为42.86%,64.29%,43.88%.NP作用后瘤组织中PCNA、bcl-2细胞的阳性率显著降低,bax细胞的阳性率显著升高,NP与环磷酰胺有一定的协同效应.
Polysaccharide from the leave of Nerium indicum Mill. was extracted by water and precipitated by ethanol, the mice S180 solid tumor and ascites tumor model was established to evaluate the anti-tumor activity of NP, the changes of expression of PCNA, bcl-2 and bax protein in tumor tissues were detected by immunohistochemical method. Enhancement of CTX anti-tumor effects by NP was explored further by coefficient of drug interaction. The results demonstrated that the content of polysaccharide is 81.14% determined by phenol-sulfuric acid method analysis. NP inhibited Sis0 sarcoma growth in mice and prolonged the life-span of S180-ascites tumor mice evidently. The tumor inhibition rates （IR） were 40. 00%, 50. 83%, 44.17% and the life-span extension rates （ER） were 42.86%, 64.29%, 43.88% after 50, 100, 150 mg/kg NP-ip. The positive rate of PCNA, bcl-2 in tumor tissues were decreased, and bax were enhanced remarkably in S180 solid tumor-bearing mice by NP treated. There was synergistic effect in the cooperation of NP and CTX.
出处
《福建师范大学学报（自然科学版）》
CAS
CSCD
北大核心
2009年第4期95-99,共5页
Journal of Fujian Normal University：Natural Science Edition
基金
福建省科技厅基金资助项目(2008F5018)
关键词
夹竹桃
多糖
S180
抑瘤作用
免疫组化
Nerium indicum Mill.
polysaccharide
S180
anti-tumor
immunohistochemistry
