摘要
采用毛细管电泳技术建立了血清蛋白毛细管自由区带电泳分析方法，并与醋酸纤维薄膜电泳法相比较。利用ｐＨ９．８、１００ｍｍｏｌ／Ｌ硼酸缓冲液作电泳缓冲液，未涂渍毛细管２１ｃｍ×５０μｍ（ｉ．ｄ），检测波长２００ｎｍ，当电压为０．５ｋＶ／ｃｍ时，在５分钟内可将血清蛋白分为五条蛋白区带。利用ｐＨ８．５、２５ｍｍｏｌ／ＬＳＤＳ、２０ｍｍｏｌ／Ｌ硼酸缓冲液作样品稀释液，未涂渍毛细管４５ｃｍ×５０μｍ（ｉ．ｄ），在电压２２．５ｋＶ条件下，可将血清蛋白分离１０条区带。该方法稳定、重复性好。
This study details the qualitative results from a comparative evaluation of the separation of human serum proteins by cellulose acetate electrophoresis and free zone capillary electrophoresis.When the conditions for the separation of serum proteins were set at 100 mmol/L borate buffer at pH 98 in an untreated 50 μm(id)×21cm(16 cm to detector)capillary with detection wavelength of 200 nm.Five serum proteins were separated by the capillary electrophoresis in less than 5 minutes.The method had execellent reproducibility.Ten protein peaks were detected when a diluent containing 25 mmol/L sodium dodecyl sulfate (SDS),20 mmol/L borate buffer at pH 85 was used to dilute the serum samples in a 45 cm×50 μm(id)fusedsilica capillary(40 cm to detector)with a running voltage of 225 kVIn conclusion,the rapid analysis,execellent reproducibility of this automated capillary zone electrophoresis(CZE)method for the separation of serum proteins appears to be suitable for use in the routine laboratories in a clinical setting.
出处
《临床检验杂志》
CAS
CSCD
北大核心
1998年第4期203-205,共3页
Chinese Journal of Clinical Laboratory Science
