摘要
目的探讨泛耐药铜绿假单胞菌(PDRPA)β-内酰胺酶基因存在状况和菌株亲缘性。方法应用PCR检测21种β-内酰胺酶基因和整合子、转座子遗传标记。结果33株PDRPA blaTEM、blaOXA-10群、blaPER、blaGES、blaCARB、intⅠ1、merA基因阳性率分别为48.5%、45.5%、33.3%、21.2%、15.2%、78.8%、69.7%;聚类分析示存在克隆传播现象。结论PDRPA blaTEM、blaOXA-10群、blaPER、blaGES、blaCARB、intⅠ1、merA基因检出率高,PDRPA可存在克隆传播医院感染。
OBJECTIVE To investigate the presence of β-lactamase genes in pan-drug resistant Pseudomonas aeruginosa （PDRPA）, and the redateness in PDRPA. METHODS The genes of 21 kinds of resistance were detected by polymerase chain reaction and phylogenetic analysis. RESULTS In 33 strains of PDRPA, the positive rates of blaTEM,blaOXA-10cluster, blaPER,blaGES,blaCARB, int Ⅰ 1 and merA were 48.5 %, 45.5%, 33.3%, 21.2%, 15.2% ,78. 8% and 69.7%, respectively. There were clone transmitted phenomena. CONCLUSIONS There are very high positive percentages of blaTEM, blaOXA-10cluster, blaPER, blaGES, blaCARB, int Ⅰ 1 and merA genes in PDRPA. The PDRPA can induce clone transmitted hospital infection.
出处
《中华医院感染学杂志》
CAS
CSCD
北大核心
2009年第13期1621-1623,共3页
Chinese Journal of Nosocomiology
关键词
泛耐药铜绿假单胞菌
Β-内酰胺酶
亲缘性分析
Pan-drug resistant Pseudomonas aeruginosa
β-Lactamase
Phylogenetic analysis
