摘要
目的探讨新生大鼠脑白质损伤时GRP78和CHOP基因表达变化及意义。方法2日龄SD大鼠98只,实验组和对照组各49只,实验组大鼠制备脑白质损伤(WMD)动物模型,两组均于缺氧缺血后0、2、4、6、12、24 h及72 h处死,HE染色观察脑组织病理学变化,real time PCR技术检测GRP78mRNA及CHOPmRNA表达量变化。结果与对照组相比,实验组GRP78mRNA在缺氧缺血后2h表达开始上升,6 h达到峰值,缺氧缺血后2、4、6、12、24、72 h均增加(P<0.05)。实验组CHOPmRNA在缺氧缺血后2 h开始表达上调,逐渐上升,缺氧缺血后2、4、6、12、24、72 h均增加(P<0.05)。结论新生大鼠脑白质损伤时,实验组GRP78mRNA及CHOPmRNA表达较对照组显著升高,且两个基因表达升高有时序性。表明缺氧缺血导致内质网应激反应被激活,内质网应激可能是新生大鼠脑白质损伤的发病机制之一。
Objective To study the changes of GRP78 and CHOP in neonate rats with hypoxia-ischemic white matter damage（WMD） experimentally and discuss the significance of these changes.Methods Ninety-eight 2nd-day SD rats were randommized to experimental group and control group.The neonate rats,were perfused at 0,2,4,6,12,24 and 72 h of recovery from hypoxic-ischemia.The light microscope was used to observe the brain for pathological changes after hypoxia and ischemia（HI）.Real time PCR was used to detect the expression of GRP78mRNA and CHOPmRNA in the white matter tissue of both groups. Results The expression of GRP78mRNA up-regulated at 2 h after WMD, peaked at 6 h, demonstrating significant differences at 2, 4, 6, 12, 24 h and 72 h compared with the control group （P 〈 0. 05 ）. The level of CHOPmRNA up-regulated at 2 h after WMD, demonstrating significant differences at 4, 6, 12, 24 and 72 h （P 〈0. 05） compared with that in the control group. Conclusion In the WMD neonatal rat, the time dependant expression of GRP78 and CHOP increased significantly compared with the control group. Endoplasmic reticulum stress was induced during hypoxia-ischemia.Endoplasmic reticulum stress seems to be involved in the apoptosis of oligodendrocyte induced by hypoxia-ischemia.
出处
《中国新生儿科杂志》
CAS
2009年第3期161-164,193,共5页
Chinese Journal of Neonatology
