摘要
分别采用固体培养基和液体培养基对经过携带目的基因的农杆菌转化的枇杷花药胚状体进行培养。结果表明:液体培养在转速为100 rpm时胚状体的生长和增殖最佳;选择培养采用的抗菌素以安必西林1000 mg/L+卡那霉素100 mg/L为宜;液体培养基上产生次生胚的百分率和平均产生的次生胚数都高于固体培养基。液体培养获得的增殖胚发育的时期一致性较高,生活力旺盛;固体培养获得的增殖胚状体多数成簇状分布,容易提早萌发。
Liquid medium and solid medium were applied to culture loquat anther-derived embryos transformed mediated with Agrobacterium tumefaciens. Results showed：when the Shaker worked with 100 rpm,the embryos grew best;in selection medium 1 000 mg/L ampicilin and 100 mg/L Kanamycin was the optimal antibiotic concentration to select the loquat anther-derived embryos after transformation; the percentage of embryos producing new embryos and No. of newly formed embryos per embryo producing new embryos in liquid medium both higher than that of solid medium. In liquid medium most of the newly formed embryos developed at the same stage and grew.ed well.
出处
《北方园艺》
CAS
北大核心
2009年第7期104-106,共3页
Northern Horticulture
关键词
枇杷
花药胚状体
液体培养
遗传转化
Loquat
Anther-derived embryo
Liquid culture
Transformation
