摘要
研究胃安胶囊中芍药苷含量测定的方法。采用反相高效液相色谱法,ODS-C18色谱柱（4.6 mm×250 mm,5μm）;以乙腈-0.2%磷酸溶液（15∶85）为流动相;流速为1.0 mL/min;检测波长230 nm;柱温30℃。结果表明,芍药苷在5.72~57.2μg/mL的范围内与峰面积呈良好的线性关系（r=0.999 8）,平均回收率为98.69%,RSD为2.94%（n=6）。该方法简便、准确、重现性好,适用于胃安胶囊的含量测定和质量控制。
Study on the method for determination of paeoniflorin in weian capsule by RP-HPLC. The analytical column was Hypersil ODS-C18 （5 um,4.6 mm × 250 mm） filled with at 5um stationary phase, with acetonitrile-0.2% phosphoric acid solution （ 15 ： 85 ） as mobile phase and the column temperature was 30 ℃, the detection wavelength was 230 nm and the flow rate was 1.0 mL/min. The calibration curve was linear over the range of 5.72 - 57.2 ug/mL（ r = 0.999 8 ）, with the average recoveries of 98.69% （ n = 6）, RSD was 2.94%. The method is simple, accurate and stable, it was feasible for the determination of content,it can be used to control quality of Weian capsule.
出处
《应用化工》
CAS
CSCD
2009年第6期898-900,共3页
Applied Chemical Industry
关键词
芍药苷
胃安胶囊
含量测定
paeoniflorin
Weian capsule content
determination
