摘要
目的研究Toll样受体2(TLR2)和TLR4在不同皮肤炎症的表达是否有区别。方法分别外涂弗博酯、oxazolone或胶带法于裸鼠躯干部制作皮炎模型。用免疫组化的方法观察TLR2和TLR4在真皮和表皮的表达。结果皮炎上调TLR2在表皮的表达及TLR4在真皮的表达;分别外涂弗博酯及oxazolone所诱发的皮炎中炎症细胞浸润较为明显,其TLR2和TLR4的表达增强也较明显;胶带法所诱导的皮炎中其炎症细胞浸润较轻,TLR2和TLR4的表达也较轻。结论皮炎分别上调皮肤TLR2和TLR4在真皮和表皮的表达;TLR2表达增强的程度与炎症程度有关。
Objective To determine whether cutaneous TLR 2 and 4 expression vary with dermatitis types. Methods Dermatitis models were established by topical application of either tumor promotion agent （ phorbol ester）, oxazolone, or repeated tape-stripping. Cutaneous expression of TLR 2 and 4 was assessed by immunohistochemistry methods. Results Cutaneous inflammation up-regulated epidermal TLR 2 and dermal TLR 4 expression regardless of the methods inducing inflammation. A greater increase in TLR expression was observed in dermatitis induced by either phorbol ester or oxazolone, which exhibited more inflammatory cell infiltration. In contrast, a minor increase in TLR expression was seen in dermatitis model induced by tape-stripping, which showed less inflammatory cell infiltration. Conclusion Cutaneous inflammation differentially up-regulates TLR 2 and 4 expressions in epidermis and dermis. And the extent of increased TLR2 expression is determined by the severity of inflammation
出处
《中国皮肤性病学杂志》
CAS
北大核心
2009年第7期390-392,共3页
The Chinese Journal of Dermatovenereology
基金
贵州省攻关课题[黔科合NY字(2006)3046]
