摘要
目的:检测肾缺血/再灌注大鼠尿液胱抑素C含量,探讨其在缺血/再灌注急性肾损伤早期评估中的作用。方法:选取雄性SD大鼠,随机分为4组,建立缺血/再灌注急性肾损伤动物模型,缺血时间4组分别为0、10、20、30min,测定各组大鼠术前及再灌注24h后尿液胱抑素C,血清肌酐(Scr)、尿素氮(BUN)浓度,计算24h肌酐清除率(Ccr),取各组再灌注24h后肾组织作组织学检查,行肾小管坏死半定量评分。结果:各组大鼠基线肾功能差异无统计学意义,再灌注24h后与基线值相比,肾缺血0min组及10min组BUN、Scr及Ccr无显著改变;肾缺血20min组BUN、Scr无显著改变,但Ccr显著降低;肾缺血30min组BUN[(45.3±14.6)vs(13.8±1.6)mmol/L]、Scr[(160.8±22.2)vs(36.9±7.9)μmol/L]显著升高,Ccr显著降低[(1.87±0.3)vs(0.56±0.1)ml/min]。20min组及30min组肾小管坏死评分与0min组相比显著升高。再灌注24h后与基线值相比,肾缺血0min组尿液胱抑素C水平无显著改变,肾缺血10min[(0.79±0.11)vs(0.25±0.02)μg/L]、20min[(1.23±0.35)vs(0.30±0.05)μg/L]及30min组[(1.33±0.51)vs(0.28±0.03)μg/L]尿液胱抑素C水平显著升高。结论:尿液胱抑素C测定可望成为缺血/再灌注急性肾损伤的早期诊断标记物。
Objective： To determine the urinary cystatin C in rats of ischemia/repertusion（I/R） acute kidney injury,and to estimate their effects on early diagnosis. Methods： Male SD rats were selected and divided into 4 groups for I/R injury model estab- lishment. The time for bilateral renal artery occlusion were 0,10,20 and 30 min respectively. The urine cystatin C, serum creatinine （Scr） and BUN of baseline and 24 h after reperfusion were detected. The kidney histological examination 24 h after reperfusion was performed. Results. The basline values of renal function in 4 groups were not different statistically. Compared with those at baseline, at 24 h after reperfusion,BUN,Scr and Ccr did not change significantly in ischemia 10 min group and BUN,Set in 20 min group. Ccr decreased significantly in 20rain group. In 30 min group, BUN [ （45.3 ± 14.6） vs （ 13.8 ± 1.6） mmol/L], Scr[ （ 160.8 ± 22.2） vs （36.9 ± 7.9）pxnol/L] increased significantly, and Ccr decreased significantly [ （ 1.87 ± 0.3） vs （0.56 ± 0.1 ）ml/min]. The urinary cystatin C levels were increased significantly in 10 min group [ （ 0.79 ± 0.11 ） vs （ 0.25 ± 0.02） μg/L], 20 min group [ （ 1.23 ± 0.35） vs （0.30 ± 0.05）μg/L] and 30 min group [ （ 1.33 ± 0.51 ） vs （0.28 ± 0.03）μg/L] compared with throe at baseline. Conclusion.The determination of urinary cystatin C is usefull for early diagnosis of I/R acute kidney injury.
出处
《中国中西医结合肾病杂志》
2009年第7期570-572,I0001,共4页
Chinese Journal of Integrated Traditional and Western Nephrology
基金
第二军医大学青年启动基金资助项目(No.07QN27)
