摘要
目的研究脂多糖(lipopolysaccharide,LPS)刺激肺泡巨噬细胞(alveolar macrophage,AM)后,在不同的时间点髓系细胞触发受体1(triggering receptors expressed on myeloid cells1,TREM1)、髓系细胞触发受体2(TREM2)蛋白的表达情况,并探讨其在AM介导的炎症反应中的作用及机制。方法体外培养肺泡巨噬细胞,分为正常对照组和LPS干预组。对照组不予处理,干预组给予100ng/ml LPS。之后对照组在0h,LPS干预组分别在3h,6h,12h,24h用ELISA检测AM培养上清液中TNF-α表达水平,流式细胞术检测上述不同时间点TREM1,TREM2蛋白的表达水平。结果LPS干预AM后,TNF-α的表达在3h达到最高[(36.58±2.14)pg/ml],之后下降,24h时接近正常。TREM1的表达在3h上升到最高(113.19±10.14),之后下降,24h时接近正常。TREM2的表达3h下降到最低(119.74±2.58)。TREM1与TNF-α的表达高度正相关(r=0.825,P<0.001);TREM2与TNF-α的表达高度负相关(r=-0.779,P<0.001)。结论TREM1,TREM2可能在LPS致AM介导的炎症反应中起重要作用。
Objective To investigate the expression of TREM1 and TREM2 at different time points after LPS stimulating the alveolar macrophages （ AM）, and to explore its role in AM-mediated inflammatory response. Methods The alveolar macrophages were cultured and divided into control group and LPS group. The alveolar macrophages were added nothing in control group, while added LPS at the final concentration of 100 ng/ml in LPS group. Then the expression level of TNF-α in the supernatant fluid of alveolar macrophages was detected with ELISA at 3 h ,6 h, 12 h ,24 h after LPS stimulation in LPS group and at 0 h in control group, and the expression of TREM1 and TREM2 were simultaneously detected with flow cytometry（FACS）. Results After LPS stimulating AM,the expression of TNF-α peaked at 3 h [ （36.58 ± 2. 14）pg/ml ], and then decreased to the normal at 24 h. The expression of TREM1 also peaked at 3 h （ 113.19 ± 10.14） , and decreased to the normal at 24 h. TREM2 expression fell to the lowest（ 119.74 ± 2.58） at 3 h, and then rose. TREM-1 expression was positively correlated with TNF-α expression （ r = 0. 825, P 〈 0.001 ）, while TREM-2 expression was negatively correlated with TNF-α expression（r = -0.779,P 〈0. 001 ）. Conclusion TREM1 and TREM2 may play an important role in AM-mediated inflammation response.
出处
《山西医科大学学报》
CAS
2009年第7期580-582,616,共4页
Journal of Shanxi Medical University
关键词
髓系细胞触发受体1
髓系细胞触发受体2
肺泡巨噬细胞
脂多糖
triggering receptors expressed on myeloid cells 1
triggering receptors expressed on myeloid cells 2
alveolar macro-phages
lipopolysaccharide
