摘要
探讨了附桂骨痛复方中主要有效成分乌头类总生物碱的质量浓度测定方法。依据乌头类总生物碱与溴甲酚绿在pH=3.5的柠檬酸-磷酸氢二钠缓冲液中反应生成离子对化合物的光谱特征,利用分光光度法于412.5nm波长处测定吸收度并计算含量。结果表明,乌头碱含量在10.12～22.77μg/mL范围内与吸收度线性关系良好(r=0.9996,n=6),其平均加样回收率为99.27%。该法用于测定附桂骨痛复方提取液中乌头类总生物碱的含量准确可靠、简便易行、专属性强。
A method for the determination of aconitine-type alkaloids, the main effective components in the compound prescription Fugui Gutong, has been developed. The alkaloid content was determined by acid dye colorimetry, based on the spectrometric features of the ion-pair compounds produced in the reaction between the aconitine-type alkaloids and bromcresol green in pH 3.5 citric acid-disodium hydrogen phosphate buffer solution, with the absorbance being measured at a wavelength of 412.5 nm. The linearity range was 10.12 - 22.77μg/ mL （r = 0. 9996, n =.6）. The average recovery of total alkaloids was 99.27 %. The method is accurate, reliable, and easy to carry out and shows high selectivity for the determation of the content of aconitine-type alkaloids in Fugui Gutong.
出处
《北京化工大学学报（自然科学版）》
CAS
CSCD
北大核心
2009年第4期80-83,共4页
Journal of Beijing University of Chemical Technology(Natural Science Edition)
基金
北京联合大学生化学院生物化工重点建设学科项目(51102630815)
北京市教育委员会科技发展计划(KM200611417013)
关键词
乌头碱
溴甲酚绿
附桂骨痛
aconitine
bromocresol green
Fugui Gutong
