摘要
目的探讨肿瘤细胞接受不同剂量高能X线照射后,细胞表面MICA抗原表达及对NK细胞功能的影响。方法分别取T淋巴细胞株T2、结肠腺癌细胞株LS-174-T及食管鳞癌细胞株ECA-109,经0、4、8、163、2 Gy的剂量照射,16 h过夜培养,给细胞标记MICA的单克隆抗体,分析肿瘤细胞平均荧光密度(MFI的变化。并将外周血单个核细胞与经优化剂量照射的肿瘤细胞按10∶1比例孵育,4 h后利用流式细胞术胞内染色法分析NK细胞释放穿孔素的情况以评价NK细胞的杀伤毒性。结果不同肿瘤细胞株上调MICA表达的最佳放疗剂量不同,而肿瘤细胞上调MICA表达后可增强NK细胞的杀伤毒性。结论适宜的放射剂量促进肿瘤细胞表达MICA抗原而增强机体免疫功能。
Objective To investigate the effects of MICA expression on different tumor cell lines by a variety of doses of irradiation and the corresponding activation of NK cells. Methods Three cell lines, T2 （T cell lymphoma）, LS-174-T （colon cancer）, ECA-109 （esophageal squamous cell cancer）, were all irradiated with the increased doses of 0, 4, 8, 16, 32 Gy..Ajter overnight cultivation, those cells were labeled with MICA monoclonal antibody and detected the mean fluorescence intensity （MFI） on tumor cells by flow cytometry. In addition, the peripheral blood mononuclear cells were mixed with the optimal irradiated tumor cells with a ratio of 10 ： 1. Four hours later, perforin positive NK cells were analyzed by intracellular staining with anti-CD56 and anti-perforin monoclonal antibodies. The percentage of NK cells with secretion of perforin were calculated and regarded as a parameter of cytotoxicity. Results The up-regulated MICA expression on different cell lines needed the different doses of irradiation. The increased expression of MICA on cell membrane could enhance the cytotoxicity of NK cells. Conclusions The optimal dose of irradiation could strengthen the immune surveillance via up - regulating MICA expression on tumor cells.
出处
《实用临床医药杂志》
CAS
2009年第5期21-23,36,共4页
Journal of Clinical Medicine in Practice
基金
国家自然科学基金资助项目(30671917)
江苏省自然科学基金资助项目(BK2004404
BK2008215)
江苏省高校自然科学基金资助项目(04KJB320162)
