摘要
目的探讨荧光硅纳米颗粒做为基因载体的可行性及其自带荧光做为一种生物标志物的易于检测性。方法用微乳液法制备荧光硅纳米颗粒,并用氯化钠对其进行表面修饰。用粒径仪观察其粒径,电位分析仪检测纳米颗粒表面Zeta电位值。用上下法检测其对SD大鼠的急性毒性,并在荧光显微镜检测其在SD大鼠主要脏器的分布情况。结果制备的硅纳米粒径在32nm左右、Zeta电位为+53.9mv、LD50大于2000mg/kg、呈红色荧光,在各大脏器均有分布,特别在脑和前列腺中有大量分布。结论该荧光硅纳米颗粒可用作基因载体。
[Objective] To probe into the feasibility of Fluorescent silica nanoparticles （FSNPs） as a gene transfer vector and analyze the easy detection of the fluorescence of FSNPs as a biology marker. [Methods] The FSNPs were prepared by microemulsion. We used NaCl to do surface modification on them. Their size was detected by a particle analyzer, and their Surface Zeta Potential was measured by an electrical Potential analysator. Its acute toxicity to the SD rats was detected by up-and-down procedure and the condition of its distribution in the main organs of theSD rats was observed with fluorescence microscope. [Results] The prepared FSNPs were 45 nmin diameter, ＋ 53.9 mv in surface Zeta potential. Its LD50 was more than 2000 mg/kg. It could emit red fluorescence and distribute in every main organs, especially, in brain and prostate. [Conclusions] The prepared FSNPs can be a gene vector.
出处
《中国现代医学杂志》
CAS
CSCD
北大核心
2009年第12期1801-1804,1808,共5页
China Journal of Modern Medicine
