摘要
建立一种采用反相高效液相色谱法测定黄酒中β-苯乙醇的方法。采用反相C18色谱柱SynergiHrdro-RPC18(4.6mm×250mm,4μm),以甲醇-水为流动相(50:50,V/V),流速1ml/min,紫外检测器,检测波长210nm,对黄酒中的β-苯乙醇进行检测。结果表明,在5.00～30.00mg/L添加量范围内,回收率水平在99.5%～99.8%之间,相对标准偏差为0.6%(n=6),方法的检出限为0.05mg/L,线性范围为0.5～50mg/L(r=0.9999),测定结果与标准气相色谱方法基本相同。所建立的方法可以作为黄酒中β-苯乙醇的检测方法。
This study developed a reverse phase HPLC method for determing 15-phenylethanol in Chinese rice wine. Beta- phenylethanol was separated on a Synergi Hrdro-RP C18 column （4.6 ×250 mm, 4 μm） using a mobile phase composed of CH3OH and H2O （50：50, V/V） at a flow rate of 1 ml/min and detected using a UV detector set at 210 nm. At the spike levels of 5.00, 15.00 and 30.00 mg/L, the average recoveries for 13 -phenylethanol were between 99.5% and 99.8% with the RSDs of less than 0.6% （n = 3）; the limit of detection was 1.0 ng, and the linear range was from 0.5 to 50 mg/L （r = 0.9999）. The results of β - phenylethanol determination in four different rice wine samples by this method were almost the same as those determined by gas chromatography method. Therefore, the method developed can be use to determine β-phenylethanol in Chinese rice wine.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第14期175-177,共3页
Food Science
基金
国家质量监督检验检疫总局科技项目(2005QK124)
