摘要
用高效液相色谱法测定奶及乳制品中三聚氰胺的含量。色谱条件为:色谱柱:C18柱(150mm×4.6mm,5μm);流动相:乙腈:0.202%庚烷磺酸钠-0.210%柠檬酸水溶液(10:90,V/V);检测波长:235.8nm;柱温:30℃。结果表明:在0.1～1.6μg/ml范围内三聚氰胺与峰面积呈良好线性关系,相关系数为0.9973,回归方程为A=59746C+183.48,回收率为98.3%～99.2%,相对标准偏差小于1.52%。
A HPLC method was developed for determining melamine in milk and dairy products. The analysis was carded out using the Waters HPLC system equipped with a Cls column （150 mm ×4. 6 mm, 5 μm） and a diode array detector sex at 235.8 nm. The mobile phase consisted of 0.202% haptane sodium sulphate acetonitrile solution and 0.210% citric acid aqueous solution （10：90, V/V） with a flow rate of 1.0 ml/min, and the column temperature was set at 30 ℃. A good linear correlation （the regression equation： A = 59746C ＋ 183.48） between the peak area and the melamine determination was found in the range between 0.1 and 1.6 μg/ml with a correlation coefficient of 0.9973. The recoveries for melamine in three spiked dairy products ranged 98.3%- 99.2% with a RSD of less than 1.52 %.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2009年第14期210-212,共3页
Food Science
基金
辽宁省教育厅科学技术基金资助课题(20331079)
