期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

RNAi在医学实验动物研究中的应用

Application of RNAi in Medical Laboratory Animals
下载PDF
导出
摘要 RNAi的发现使基因功能的研究和人类疾病的治疗有了新的途径,特别是在抗病毒、抗肿瘤研究中具有较高的应用前景,同时在心血管疾病、神经系统疾病、内分泌系统疾病等研究中也将发挥重要作用。RNAi将会是医学实验动物模型建立与研究的有力工具。 The discovery of RNAi made a new way for the study on gene function and therapy of human diseases. There is a prospect of the application in antiviral therapy and anti tumor research, and play an important role in research of cardiovascular diseases, nervous system disease, and endocrine system disease. RNAi will be a tool for construction and research of the medical laboratory animal model.
作者 鲁帅尧 和占龙 禹文海 赵远 谭德勇
机构地区 中国医学科学院北京协和医学院医学生物学研究所 云南大学
出处 《中国比较医学杂志》 CAS 2009年第7期60-63,共4页 Chinese Journal of Comparative Medicine
关键词 RNAI 基因工程动物 疾病 基因治疗 RNA interference Genetic engineering of animal Disease Gene therapy
分类号 R-33 [医药卫生]
  • 相关文献

参考文献24

  • 1Fire.A,Xu S,Montgomery MK,et al.Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans[J].Nature,1998,391(6669):806-811.
  • 2Aravin AA,Klenov MS,Vagin VV,et al.Role of double2st randed RNA in eukaryotic gene silencing[J].Mol Biol,2002,36(2):180-188.
  • 3鲁艳明,张淑兰.RNAi-基因敲除技术在妇科领域中的应用[J].国外医学（妇幼保健分册）,2005,16(3):186-189. 被引量：1
  • 4刘杰凤,王志辉,李春晖.RNA干扰用于基因治疗的研究进展[J].生物技术通讯,2006,17(2):276-278. 被引量：4
  • 5McMichael AJ,Rowland-Jones SL.Cellular immune responses to HIV[J].Nature,2001,410:980-987.
  • 6Myslinski E,Ame JC,Krol A,et al.An unusually aompact external promoter f or RNA polymerase transcription of the human H1RNA gene[J].Nucleic Acids Res,2001,29(12):2502-2509.
  • 7Elbashir SM,lendeckel W,Tuschl T,et al.Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cell[J].Nature,2001,411(6836):428-439.
  • 8Jacque JM,Triques K,Stevenson M.Modulation of HIV-1replication by RNAi[J].Nature,2002,418:435-438.
  • 9Kimpton J,Emenrman M.Detection of replication-competent and pseudotyped human immunodeficiency virus with a sensitive cell line on the basis of activation of an integrated bgalactesidase gene[J].J Virol,1992,66:2232-2239.
  • 10McCaffrey AP,Nakai H,Pandey K,el al.Inhibition of hepatitis B virus in mice by RNA interference[J].Nat Biotochnol.2003,21:639-644.

二级参考文献18

  • 1朱旭东,党颖,冯怡,李涛,黄培堂.用RNaseⅢ制备的小干涉RNA降解SARS冠状病毒基因的细胞内转录物[J].生物工程学报,2004,20(4):484-489. 被引量：5
  • 2林剑萍,于永利,王丽颖.针对SARS冠状病毒S蛋白的RNAi设计[J].生物技术通讯,2004,15(4):335-337. 被引量：5
  • 3Aravin A A, Klenov M S, Vagin V V, et al. Role of double-stranded RNA in eukaryotic gene silencing[J]. Mol Biol,2002,36(2):180-188.
  • 4Myslinski E, Ame J C, Krol A, et al. An unusually aompact external promoter for RNA polymerase Ⅲ transcription of the human H1RNA gene [J].Nucleic Acids Res, 2001,29(12):2502-2509.
  • 5Elbashir S M,lendeckel W,Tuschl T,et al.Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cell[J]. Nature,2001,411(6836) :428 -439.
  • 6Brummelkamp T R, Bernards R, Agami R. A system for stable expression of short interfering RNAs in mammalian cells [J].Science,2002,296期:550 -559.
  • 7Sui G S, Soohoo C, Affar E B. A DNA vector-based RNAi technology to suppression gene expression in mammalian cells[J]. PNAS,2002,99(8) :5515-5521.
  • 8Hall A H, Alexander K A. RNA interference of human papillomavirus type 18E6 and E7 ineluces senescence in Hela cells[J]. J Virol,2002,77(10):6066 6079.
  • 9Wang Y, Decker S J, Sebolt L J, et al. Knockdown of Chkl, Weel and Mytl by RNA Interference Abrogates G (2)Checkpoint and Induces Apoptosis[J]. Cancer Biol Ther,2004,3(3):308 - 317.
  • 10Milner J. RNA interference for treating cancers caused by viral infection[J]. Expert Opin Biol Ther,2003,3(3):459- 467.

共引文献3

中国比较医学杂志
2009年 第7期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部