摘要
目的:探讨血清同型半胱氨酸水平和5,10-亚甲基四氢叶酸还原酶基因多态性与无症状性脑梗死的关系。方法:用酶联免疫吸附法测定68例无症状性脑梗死患者和62例健康对照者血清同型半胱氨酸水平,应用聚合酶链反应-限制性片段长度多态性技术对68例无症状性脑梗死患者和62例健康对照者进行亚甲基四氢叶酸还原酶基因多态性分析。结果:无症状性脑梗死组血清同型半胱氨酸水平[(23.24±6.42)μmol/L]显著高于健康对照组[(14.98±3.64)μmol/L](P<0.01)。无症状性脑梗死组中突变纯合子TT基因型频率42.6%,突变杂合子CT基因型频率44.1%,野生型CC基因型频率为13.2%;健康对照组中TT基因型频率为33.9%,CT基因频率型为30.6%,CC基因型频率为35.5%;无症状性脑梗死组和健康对照组T等位基因频率分别为64.7%和49.2%,C等位基因型频率分别为35.3%和50.8%,以上各频率之间比较差异均有统计学意义(P<0.05)。结论:高同型半胱氨酸血症是无症状性脑梗死的一个独立危险因素,5,10-亚甲基四氢叶酸还原酶基因可能是无症状性脑梗死的易感基因。
Objective To explore the relationship of serum homocysteine levels and methylenetetrahydrofolate reductase gene polymorphisms with silent cerebral infarction. Methods The 68 patients with silent cerebral infarction and 62 cases of healthy controls were recruited in the study. Their serum homocysteine levels were measured by ELISA, and their polymorphisms of methylenetetrahydrofolate reductase gene were analyzed with polymerase chain reaction-restriction fragment length polymorphism. Results Serum homocysteine levels in patients with silent cerebral infarction [（23.24±6.42） μmol/L] was significantly higher than that [（14.98±3.64） μmol/L] in healthy control group （P〈0.01）. The frequency of homozygous mutant TT-type was 42. 6%,the frequency of heterozygous mutant CT-type was 44. 1%, and the frequency of wild mutant CC-type was 13.2% in patients with silent cerebral infarction. The frequencies of TT-type, CT-type and CC- type were 33.9%, 30.6% and 35.5%0 ,respectively in control group. The frequencies of T alleles in silent cerebral infarction group and control group were 64.7% and 49.2%, and the frequencies of C alleles in silent cerebral infarction group and control group were 35. 3% and 50. 8%, respectively. There were statistic differences between the above frequencies （ P 〈 0. 05 ）. Conclusion Hyperhomocysteinemia is an independent risk factor for silent cerebral infarction. Methylenetetrahydrofolate reductase gene may have a role in genetic susceptibility for silent cerebral infarction.
出处
《中华实用诊断与治疗杂志》
2009年第7期634-636,共3页
Journal of Chinese Practical Diagnosis and Therapy
基金
河南省基础与前沿技术研究计划项目(082300450370)
河南省医学科技攻关计划项目(2006)
郑州市科技攻关计划项目(064SGYS33216-2)
