摘要
道地中药材茅苍术具有广泛的形态多样性。为了识别其遗传多样性,应用ISSR分子标记技术对16个茅苍术基因组和4个北苍术基因组进行了多态性分析。结果发现,茅苍术ISSR多态位点百分率为87.67%,北苍术ISSR多态位点百分率为53.33%,证明茅苍术比北苍术有更高的遗传多样性。采用SPSS13.0软件的Within-group linkage法对20个样品进行聚类分析。结果表明,茅苍术具有明显的遗传分化,且与北苍术有很近的亲缘关系。
Atractylodes lancea, now in Chinese, is a very important famous region-drug. As a wild speeies,Atractylodes lancea is endemic to Jiangsu province. ISSR analysis was conducted on 20 samples （16 samples of Atractylis lancea genome and 4 samples of Atractylis chinensis genome） to investigate the genetic diversity. For Atractylis lancea, the polyrnorphic locus percentage is 87.67%, while for Atractylis chinensis, 53.33%; which indicated that the levels of genetic diversity existed in Atractylodes lancea was higher than that in Atractylodes chinensis. Otherwise, the 20 samples were performed cluster analysis using within-group linkage method in SPSS 13.0 software. The analysis indicated that for Atractylis lancea there is obvious genetic differentiation,and there is close affiliation between Atractylodes lancea and Atractylodes chinensis.
出处
《河南农业科学》
CSCD
北大核心
2009年第7期90-93,共4页
Journal of Henan Agricultural Sciences
基金
江苏省高技术研究项目(BG2006322)
镇江市农业项目(NY200645)
关键词
茅苍术
ISSR
遗传多样性
Atractylodes lancea
ISSR
Genetic diversity
