摘要
目的选择一种建立长波紫外线(ultrayiolet,UVA)照射人表皮角质形成细胞损伤模型的理想载物界面。方法选取原代培养的处于指数生长期的角质形成细胞,分别采用泡沫板、锡纸板、冰面作为载物界面,进行相同剂量的UVA照射,同时设未照射组为对照组,通过改良MTT法检测细胞损伤程度。结果冰面作为载物界面时,细胞损伤相对较弱,损伤率仅为19.88%;锡纸面作为载物界面时,细胞照射损伤最重,损伤率高达82.76%;泡沫板作为载物界面时的细胞照射损伤率为55.58%,介于上述两种载物界面之间,各组与对照组比较均有显著性差异(P<0.05)。结论泡沫板是UVA照射人表皮细胞损伤模型的理想载物界面。
Objective To choose a better matter interface for the models of UVA induced human epiderm keratinocytes irradiation damage. Methods To irradiate the human epiderm keratinocytes with an identical dose of UVA, which is primary cultured and is occupying exponential growth phase. The matter interface is cystosepiment, tin foil and ice. The non-irradiation group is the control group. To detect the cells degree of injury through the improved methyl thiazolyl tetrazolium （MTT） assay. Results The frequency of injury is 19.88% with the matter interface of ice, and cells injury is feeble. The injury rate is 82.76% with the matter interface of tin foil, and cells injury is heaviest. But the injury rate is 55.58% with the matter interface of cystosepiment, the ceils injury is between the ice and the fin foil. To compared with the control group, each group have a significant difference （P〈0.05）. Conculusion To decided that the matter interface is the cystosepiment when the period of irradiation.
出处
《中国实用医药》
2009年第5期1-2,共2页
China Practical Medicine
基金
云南省自然科学基金项目(项目编号:2004C0018Q)
