摘要
对新鲜多宝鱼微生物指标进行定性和定量分析。通过对体表的细菌进行分离、纯化后,采用细菌的传统鉴定方法与现代自动化鉴定仪器相结合的方法,对分离纯化所得到的鱼体菌种进行鉴定,完成多宝鱼的细菌菌相分析。结果表明:分离获得的206株细菌,革兰氏阳性菌占菌株总数的18.9%,革兰氏阴性菌占菌株总数的72.3%,其中的优势菌株为金黄色葡萄球菌(12.6%)、液化沙雷氏菌(10.7%)、肠杆菌属(19.4%)和莫拉氏菌属(8.3%)。其中肠杆菌的数量较多,说明养殖鱼所用海水受污染较重,应引起重视,为后期研究冷藏过程中多宝鱼的特定腐败菌及生鲜多宝鱼冷藏工艺的优化和新工艺的开发提供理论依据。
The microbiological characters and composition of bacterial flora of fresh Paralichthys Lethostigma were observed with qualitative and quantitative analysis.Bacteria from fresh Paralichthys Lethostigma skin were isolated and purified, using a combination method of the traditional and modern automated equipment identification,the bacteria from fresh fish skin were separated, purified and identified, bacteria flora analysis was completed. The results showed among 206 isolated bacteria, Gram-positive bacteria strains accounted for 18.9% of the total, Gram -negative bacteria accounted for 72.3% of the total number of strains, including advantaged strains of Staphylococcus aureus （ 12.6% ）, Liquefaciens（ 10.7% ）, Enterobacter （ 19.4% ） and Moraxella （ 8.3% ）.The results showed that there were a large number of Enterobacter in fish farm,the farming water were polluted.The research provided a theoretical basis of optimization and the development of new technology for the latter study of the specific spoilage organism from cold storage Paralichthys Lethostigma.
出处
《食品工业科技》
CAS
CSCD
北大核心
2009年第7期112-114,共3页
Science and Technology of Food Industry
基金
辽宁省高等学校优秀人才支持计划(2007R04)
关键词
养殖多宝鱼
分离鉴定
细菌菌相分析
cultured Paralichthys Lethostigma
isolation and identification
bacteria flora analysis
