摘要
目的:研究金黄色葡萄球菌在体外不同培养条件下形成生物膜的能力。方法:在不同温度、不同载体、不同培养时间及营养不同条件下进行培养,测出细菌生物膜表面积,并进行两两比较。结果:在不同温度下培养的细菌膜,25℃的表面积有显著性差异;不同的载体培养的细菌膜两两比较有极显著差异,以硅胶膜的差异最大;培养5d、6d菌膜的表面积明显高于3d的表面积;振动与非振动培养的菌膜有极显著差异。结论:在体外细菌形成细菌膜的能力与培养条件密切相关,培养温度、载体、时间和营养条件对金黄色葡萄球菌细菌膜生长都有影响。
Objects：To study the for mation ability of biofilm of staphylococcus aureus in vitro under different culture conditions. Method ： Surface areas of bacterial biofilm were tested and compared each other under culture conditions including different temperatures, carriers, culturing periods and nutrientsubstances. Results ： The results show that difference of bacteria membrane surface area was remarkable in different temperatures, especially in 25~C temperature, difference of bacteria membrane surface area was also remarkable in different carries especially in aerosil film, bacteria membrane surface areas of 5 days -cuturing were 6 days - culturing were more significant than that in 3 days culturing,and significant difference also happened in shaking and non-shaking conditions. Conclusion ： The formation ability of biofilm of staphylococcus aureus in vitro is closely related to culture conditions, different temperature, carriers, time and nutritional conditions affect on Staphylococcus aureus bacterial membrane growth.
出处
《陕西医学杂志》
CAS
2009年第7期780-782,共3页
Shaanxi Medical Journal
基金
广东省科技计划项目(2005B33001004)
