摘要
目的:观察瑞芬太尼预处理对大鼠短暂性全脑缺血再灌注损伤的影响。方法:38只大鼠随机分为5组:假手术组(n=6);缺血/再灌注前输注生理盐水组(n=8);瑞芬太尼2,6,20μg.kg-1.m in-1组(每组n=8);根据Sm ith等创建的"双侧颈总动脉阻断+低血压法"建立大鼠短暂性全脑缺血模型。瑞芬太尼在全脑缺血前30 m in由股静脉泵入,于双侧颈总动脉阻断前及开放后10 m in,从股动脉抽取0.5 mL血样,进行动脉血血气分析,于再灌注后3 d灌注取脑,并制作脑切片,HE染色,双盲法观察高倍镜下海马CA1、CA4区、纹状体、大脑皮层等脑区退变的神经细胞数,并计算出退变的神经锥体细胞的百分率。结果:各处理组生理参数变化差异无显著性;再灌注3 d后,生理盐水组及各瑞芬太尼预处理组出现显著的神经元损伤,与生理盐水组相比,芬瑞太尼6μg.kg-1.m in-1及芬瑞太尼20μg.kg-1m in.-1组海马CA1区退变的神经元较生理盐水组明显减少,各组CA4区未见明显的神经元退变细胞;再灌注3 d后,芬瑞太尼6μg.kg-1.m in-1及芬瑞太尼20μg.kg-1m in.-1组大脑皮层退变神经元较生理盐水组明显减少;与假手术组相比,生理盐水组及不同剂量的瑞芬太尼预处理较假手术组纹状体区退变神经元显著增多,但生理盐水组及各瑞芬太尼预处理组的差异无显著性。结论:瑞芬太尼预处理对全脑缺血再灌注后的神经元损伤具有保护作用。
Objective：To study the effect of remifentanil pretreatment on forebrain ischemia/reperfusion （I/R） injury in rats. Methods ：Thirty-eight male Sprague-Dawlay rats were randomly divided into 5 groups ： sham （n=6）,control（n=8）, remifentanil 2 μg·kg^-1·min^-1,6 μg·kg^-1·min^-1 and 20 μg·kg^-1·min^-1（n =8/per group）. Transient forebrain ischemia was induced by 10 rain of bilateral common carotid artery （CCA） occlusion in combination with hypotension. At 30 min before the occlusion, rats were pretreated with intravenous remifentanil 0, 2, 6 or 20μg·kg^-1·min^-1. Right femoral artery cannulated to withdraw blood samples for analysis blood gas at 10 min before and 10 min after ischemia. At 3 days after reperfusion, brains were fixed and paraffin sections were cut. The total of damaged neurons was counted in hippocampal CA1 and CA4, cortex, and striatum; the percentage of dead neurons was calculated. Results：Physiologic variables were similar in all the groups. Forebrain ischemia significantly increased the percentage of dead neurons in hippocampal CA1 at 3 days after reperfusion. Remifentanil at 6 and 20 μg·kg^-1·min^- 1 attenuated the CA1 neuron damage, but the effect of either dose was similar. No apparent neuron damage was found in hippocampal CA4 in each group. In the cortex, the number of damaged neurons was less in REM6 and REM20 groups than in ischemic control group. In the striatum, damaged neurons were increased in all the ischemic rats as compared to sham group, but there were no significant difference among rats with or without remifentanil treatment. Conclusion：Remifentanil has protective effect on neuron damage after brain I/R injury in rats.
出处
《中国新药杂志》
CAS
CSCD
北大核心
2009年第14期1333-1337,共5页
Chinese Journal of New Drugs
基金
2008上海市卫生局科研课题(编号:2008123)
关键词
哌啶类
再灌注损伤
脑缺血
大鼠
piperidines
reperfusion injury
brain ischemia
rats
