摘要
目的探讨去冷沉淀血浆中部分有效成分的实际含量,为临床选择不同血浆输注提供实验室依据。方法采集23袋(200ml/袋)ACD-B血液保存液保存的血液,4h内分离制备新鲜冰冻血浆,各袋均留2份标本;1份同新鲜冰冻血浆一起置-35℃保存,于第3天速融后检测总蛋白、白蛋白、纤维蛋白原、凝血因子Ⅴ、Ⅷ、Ⅹ含量;1份置4℃保存21d后检测总蛋白、白蛋白、纤维蛋白原、凝血因子Ⅴ、Ⅷ、Ⅹ含量;新鲜冰冻血浆于第3天取出制备冷沉淀,留取去冷沉淀血浆标本1份检测总蛋白、白蛋白、纤维蛋白原、凝血因子Ⅴ、Ⅷ、Ⅹ含量。结果去冷沉淀血浆中的血浆蛋白和凝血因子均低于普通血浆(P<0.001)。结论去冷沉淀血浆的使用价值有局限性,不能与普通血浆等同使用。
Objective To know about some of the actual amount of active ingredient in plasma cryoprecipitate reduced, so as to provide experimental evidence for different selection of plasma infusion. Method To collect 23 bags of whole hlood（ACD-B,200 ml）,and to separate and prepare fresh frozen plasma in 4 hours. Two samples were taken from each bag,one saved with the fresh frozen plasma in refrigerator of -35℃, thawed and checked total protein,albumin,fib- rinogen, factor Ⅴ ,factor Ⅷ, factor Ⅹ after3 days;the other one saved in refrigerator of 4℃, thawed and checked total protein,albumin,fibrinogen,factor Ⅴ ,factor Ⅷ, factor Ⅹ after 21 days. The fresh frozen plasma separated and prepared of cryoprecipitate after 3 days,took samples of the plasma cryoprecipitate reduced to check total protein,albumin, fibrinogen,factor Ⅴ ,factor Ⅷ ,factor Ⅹ. Results Plasma proteins and coagulation factors in plasma cryoprecipitate reduced were lower than ordinary plasma（P〈0. 001）. Conclusions The value of plasma cryoprecipitate reduced has limitations, can not be used equated with the ordinary plasma.
出处
《临床输血与检验》
CAS
2009年第3期224-226,共3页
Journal of Clinical Transfusion and Laboratory Medicine
关键词
去冷沉淀血浆
冷上清
血浆蛋白
凝血因子
Plasma cryoprecipitate reduced Cold supernatant Plasma proteins Blood coagulation factor
