摘要
目的:探讨中国人群中IL-6基因启动子中单核苷酸多态性与HBV感染的遗传易感性关联.方法:提取160例HBV感染者及212例健康献血者外周血基因组DNA,用PCR-RFLP方法检测两组人群中G-174C、G-572C和G-597A三个多态性位点的基因型.性别、吸烟、饮酒以及Hardy-Weinberg等采用Chi-square Test检测,多态性与HBV感染者风险关联及亚组关联的统计学分析采用非条件Logistic回归并同时校正混杂因素.结果:PCR-RFLP检测结果显示:IL-6基因启动子中G-174C和G-597A两个位点在中国人群中不存在多态性,而G-572C处的多态性在人群中普遍存在,其多态性位点等位基因频率分布符合Hardy-Weinberg平衡定律.统计分析表明该位点的多态性在两组人群中有明显的差异(G/C vs G/G,OR=2.65,P<0.05;C/C vs G/G,OR=3.31,P<0.05);亚组分析中表明<30岁的年龄组中差异显著(G/G vs G/C或C/C,OR=16.92,P<0.05).结论:在中国汉族人群中,IL-6基因启动子中-572处的多态性对于乙型肝炎的发生有着显著的易患关系,同时年龄对于这种易感关联有着协同作用.
AIM： To evaluate the association of IL-6 gene promoter polymorphism with the infection of hepatitis B virus （HBV） in Chinese population. METHODS： The genome DNA was obtainedfrom blood samples and three IL-6 promoter polymorphisrns （G-174C, G-572C and G-597A） were evaluated using PCR-RFLP in 160 HBV-infected patients and 212 healthy controls. Frequencies of single-nucleotide polymorphisms （SNPs） in the control group were compared with those of the HBV infection. Furthermore, subgroups of chronic carriers were compared among them. For cases and controls, the distribution of gender, age, smoking, drinking and Hardy-Weinberg equilibrium equation of the IL-6 genotype was determined using Chi-square Test; and the diversity of age using Mann-Whitney U-test. The unconditional Logistic regression model was used to analyze the statistical association of genotypes in two groups adjusted for confused factors. RESULTS： No polymorphism at position -174 and -597 was detected in all participants, but significant difference was found in the polymorphism at position -572 between the cases and controls （G/C vs G/G, OR = 2.65, P 〈 0.05; C/C vs G/G, OR = 3.31, P 〈 0.05）. Subgroups analysis, revealed the odds ratio value in the less than thirty years old group （G/G vs G/C or C/C, OR = 16.92, P 〈 0.05）. CONCLUSION： The data reveal that the polymorphism at position -572 of the IL-6 gene promoter may be associated with the susceptibility to HBV infection; meanwhile, the age is a coordinative risk factor.
出处
《世界华人消化杂志》
CAS
北大核心
2009年第15期1522-1526,共5页
World Chinese Journal of Digestology
基金
北京大学第一医院青年科研基金资助项目
No.11161~~
