摘要
结核分支杆菌H37Rv株的全基因组序列解码加速了对重组抗原的克隆和表达,LAM、38 ku抗原1、6 ku抗原、ESAT-6、CFP-10和Mtb8.4等特异性抗原的出现,弥补了经典抗原OT和PPD在实际应用中的不足,丰富了TST、LTT、ELISA和IFN-γ检测等诊断方法的内容,提高了检测的准确性。论文结合免疫学诊断抗原在各诊断方法中的应用,概括分析了各抗原的敏感性和特异性。鉴于检测经费、仪器设备和检测频率的限制,目前TST仍是实验猴场最实用的监测方法,而其他诊断方法在阳性猴的确诊和实验室研究中仍发挥着重要作用。
Since the entire genome sequence of Mycobacterium tuberculosis H37Rv was decoded, great progress has been made in cloning and expression of recombinant antigens. The LAM, 38 ku, 16 ku, ESAT- 6, CFP-10, MtbS. 4 and so on not only made up for the deficiencies of OT and PPD, but also enriched the immunodiagnostic methods such as TST,LTT, ELISA and IFN-γ assay, and improved the detection accuracy. The research progress on antigens and methods was summarized, meanwhile, the merits and disadvantages of different antigens and methods were depicted in this article. For the limit of funding, instruments and frequency, TST is still the most useful method,while the new methods play important roles in confirming the positive monkeys and lab research presently.
出处
《动物医学进展》
CSCD
北大核心
2009年第7期72-75,共4页
Progress In Veterinary Medicine
基金
广东省科技厅农业攻关项目(2006B20801004)
广东省实验动物重点实验室(2007B060101002)
关键词
实验猴
结核分支杆菌
免疫学诊断抗原
免疫学诊断方法
experimental monkeys
Mycobacterium tuberculosis
immunodiagnostic antigen
immunodiag nositic methods
