摘要
目的体外实验研究Ⅱ型胶原(CⅡ)对关节炎大鼠和正常大鼠免疫功能的影响,探讨CⅡ的免疫调节作用。方法建立大鼠胶原型关节炎模型,体外实验研究CⅡ对关节炎大鼠和正常大鼠淋巴细胞增殖反应、自然杀伤细胞(NK细胞)杀伤活性以及淋巴细胞亚群的影响。结果CⅡ在体外实验中表现为低浓度促进而高浓度抑制大鼠淋巴细胞增殖的作用,对NK细胞杀伤活性、淋巴细胞亚群无影响。结论CⅡ对关节炎大鼠有一定的免疫调节作用。
Objective To investigate the effect on immune function by type Ⅱ collagen on arthritis in rats and normal rats in vitro. Methods Each Wistar rat was immunized with 0.1 ml of emulsion containing C Ⅱ （ final concentration of 5 mg/ml） by intradermal injection at left hind paw on d 0, and it was boosted one time on day 7 with 0.1 ml. The ConA - induced proliferative response of spleen lymphoeytes, NK cell eytotoxie activity and T lymphocyte subsets were assayed. Results Type Ⅱ eollagen can promote the proliferation of lymphoeytes at low eoneentration, and it will inhibit the proliferation of lymphoeytes at high eoneenh＇ation. It had no influence on lymphocyte subsets and NK cell eytotoxie activity. Conclusion Type Ⅱ collagen may have immunoregulation effect on arthritis in rats and normal rats.
出处
《临床和实验医学杂志》
2009年第8期6-7,共2页
Journal of Clinical and Experimental Medicine
关键词
Ⅱ型胶原
类风湿关节炎
免疫调节
大鼠
rype Ⅱ collagen
Rheumatoid arthritis
Immunoregulation
Rat
