摘要
目的探讨黄芩苷对抗结核药物肝损伤的保护作用机制。方法将60只小鼠完全随机分为6组,即正常对照组,肝损伤(异烟肼+利福霉素钠)组,联苯双酯组及黄芩苷高、中、低剂量组。给药8d后,分别用光镜和电镜观察肝脏组织细胞病理学的改变情况,用免疫组织化学染色技术分析肿瘤坏死因子-α(TNF-α)的表达情况。结果黄芩苷能明显减轻肝细胞变性和坏死,炎症活动程度明显减轻(P<0.05或P<0.01);TNF-α蛋白在黄芩苷组小鼠肝脏组织内为弱阳性表达(P<0.05或P<0.01);黄芩苷3个剂量组间小鼠肝脏病理形态学及肝脏组织内TNF-α蛋白的表达均无显著性差异(P>0.05)。结论黄芩苷对抗结核药物肝损伤的保护作用可能与抑制TNF-α蛋白表达有关。
Objective To investigate the protective effects of baicalin on the hepatic injury induced by antituberculosis drugs in mice. Methods Sixty mice were divided randomly into 6 groups： normal control group, liver injury isoniazid and rifamycin sodium group, positive control group, baicalin groups （high, middle and low doses ）. The drugs were administered to mice once daily for 8 days. Light microscope and electronic microscope were used respectively to observe the histopathological changes of the hepatic cells. The expression of tumor necrosis factor -α （TNF -α） was detected by immunohistochemistry. Results Baicalin alleviated the degeneration and necrosis induced by antituberculosis drugs obviously. The level of inflammatory activity was relieved significantly （P 〈 0.05, P 〈 0.01 ）. The immunoreaetivity of TNF - α protein in liver cells was slightly stained in baicalin groups （P 〈 0.05, P 〈0.01 ）. There were no significant differences about the pathomorphology, the inflammatory activity and the express of TNF-α protein among baicalin groups （P 〉 0.05 ）. Conclusion The protective effects of baicalin on hepatic injury induced by antitubereulosis drugs may be related to inhibit TNF -α protein expression.
出处
《时珍国医国药》
CAS
CSCD
北大核心
2009年第7期1640-1642,共3页
Lishizhen Medicine and Materia Medica Research
基金
河北省中医药管理局基金课题(No.2004040)
关键词
黄芩苷
抗结核药物
肝损伤
肿瘤坏死因子-Α
免疫组织化学
Baical in
Antituberculosis drugs
Hepatic injury
Tumor Necrosis Factor - α
Immunohistochemistry
