摘要
目的:探讨KiSS-1在鼻咽癌组织中的表达及其临床意义。方法:应用逆转录聚合酶链反应(reverse transcription polymerase chain reaction,RT-PCR)检测31例鼻咽癌和14例非肿瘤性鼻咽部组织KiSS-1mRNA的表达情况,同时分析其与临床病理指标的关系。结果:KiSS-1mRNA在鼻咽癌组表达率为67.7%,非肿瘤性鼻咽部组为85.7%,两者差异无统计学意义(P>0.05),KiSS-1mRNA表达的高低与鼻咽癌患者的性别、年龄、病理类型和T分期等临床指标均无明显关系(P>0.05)。结论:KiSS-1在非肿瘤性鼻咽上皮高表达,在鼻咽癌中表达率降低。KiSS-1表达率降低与鼻咽癌的淋巴结转移有关,提示KiSS-1基因可能在鼻咽癌的转移中起重要作用。
Objective： To investigate the expression and clinical significance of KiSS-1 gene in nasopharyngeal carcinoma （NPC） tissue. Methods：KiSS-1 mRNA expression level was detected in 31 nasopharyngeal carcinoma tissues and 14 non-tumorous tissues by reverse transcription polymerase chain reaction （RT-PCR）. The relationship between NPC and clinicopathological factors was also be analyzed. Results： The positive rate of KiSS-1 mRNA in nasopharyngeal carcinoma and non-tumorous nasopharyngeal tissues was 67.7% and 85.7%, respectively. The result was no significant difference between these two groups （ P 〉 0.05 ）. No correlation was found between the expression of KISS-1 gene and other clinicopathological factors such as patient＇s sex, age, T stage （P 〉 0.05 ）. Conclusions ：KISS-1 gene is highly expressed in non-tumorous nasopharyngeal tissue while low expressed in NPC. Loss of KISS-1 expression was correlated with clinical stage and lymph node metastasis of NPC. These inclicated that KISS-1 may play an important role in metastasis of NPC.
出处
《蚌埠医学院学报》
CAS
2009年第7期579-581,共3页
Journal of Bengbu Medical College
基金
安徽省高校省级自然科学研究资助项目(kj2007B199)
关键词
鼻咽肿瘤
KISS-1
肿瘤转移
逆转录聚合酶链反应
nasopharyngeal neoplasms
KiSS-1
neoplasm metastasis
reverse transcription polymerase chain reaction
