摘要
目的研究40%四氯化碳(CCl4)致大鼠慢性肝损伤模型病理变化过程,探讨该模型的稳定性并予以完善。方法雌性Wistar大鼠60只,用随机数字表法分为两组:对照组30只,腹腔注射玉米油0.1 ml/100 g,2次/周,实验组30只,腹腔注射40%CCl4,0.1 ml/100 g,2次/周;于实验第2、4、6周染毒48 h后分别将动物处死,取肝组织作常规HE染色,光镜下观察病理变化。结果实验组染毒2周后,肝细胞脂肪变性,小叶结构破坏,少量纤维组织增生;染毒4周后,明显的肝纤维组织增生,呈肝硬化改变;染毒6周后重度肝硬化,假小叶形成。结论40%CCl使肝组织严重坏死,第6周出现明确肝硬化。
Objective To explore the pathological changes of chronic liver injury induced by carbon tetrachloride in rat. Methods Sixty female Wistar rats were divided into control group （n = 30） and experimental group （n = 30） by mean of random number table. Rats in control group were intraperitoneal injected with corn oil,0. 1 ml/100 g,twice per week ;rats in experimental group were intrapefitoneal injected with 40% carbon tetraehloride 0. 1 ml/100 g,twice a week. They were sacrificed at 48 hours after poisoning in the 2nd,4th and 6th week respectively,then the liver were taken out and sections stained by hematoxylin-eosin,the sections were examined under light microscope. Results Ofthe liver tissue sections in experimental groups, fatty degeneration and breakage as well as little fibroustissue hyperplasia of liver lobule were observed after poisoning in the 1st week. By the 4th week, cirrhosis of liver was observed. The appearance of serious liver cirrhosis and formation of pseudolobule were observed at the 6th week. Conclusion After poisoning with 40% carbon tetrachloride,the liver injuries get severer and liver cirrhosis are observed in 6th week.
出处
《广西医学》
CAS
2009年第7期922-924,共3页
Guangxi Medical Journal
基金
广西科学基金(桂科自0832136)
关键词
四氯化碳
大鼠
慢性肝损伤
病理变化
Carbon tetrachloride
Rat
Chronic liver injury
Pathological change
