摘要
为探讨茶叶糖蛋白对小鼠骨髓来源树突状细胞（BMDCs）表面分子表达的影响，采用细胞因子诱导法，以贴壁法获得贴壁单核细胞，添加重组粒细胞-巨噬细胞集落刺激因子（rmGM—CSF）和重组白细胞介素4（rmIL-4）进行体外诱导培养，倒置显微镜动态观察细胞形态的变化；采用MTT法，观察不同浓度的茶叶糖蛋白（TGP）对小鼠骨髓来源树突状细胞的细胞毒性；采用流式细胞术检测DCs的表面标志CD11c、CD86和MHCⅡ类分子表达的变化。结果表明，经TGP作用24h后，树突状细胞形态更加典型，更加成熟；茶叶糖蛋白在（0．8—200μg／mL）的浓度范围内，可认为TGP对树突状细胞无细胞毒性。与阴性对照相比，茶叶糖蛋白显著促进树突状细胞表面CD11c、CD86、MHCⅡ的表达，在浓度（0．1～25μg／mL）范围内呈现剂量依赖性，初步表明茶叶糖蛋白可以促进树突状细胞的成熟。
The effects of tea- glycoprotein （TGP） on the surface molecules expression level of murine bone marrow derived dendritic cells were observed in our study. Dendritic cells （DCs） generated from Balb/c murine bone marrow cells were induced by rmGM - CSF and rmIL- 4. TGP was added to cells on day 6 of culture for 24 h. Morphology development of DCs was observed by invented microscope. The cytotoxicity of TGP were tested by using MTT method. The cells were analyzed by flow cytometry （FCM） to determine the proportion of CD11c cells and the change of CD86 and MHC class II molecule. The resuhs showed that TGP treated DCs displayed a mole matured morphology,with long protrusions,while untreated DCs displayed shorter protrusions than stimulated DCs. When the concentration was below 200 μg/mL, TGP showed no cytotoxixity. TGP treated immature DCs showed an enhanced cell - surface expression of CD86 and MHC Ⅱ on CD11 c gated DCs, and at the concentration （0.1 - 25 μg/mL） was in a dose -dependent upcells. regulation. TGP could induce maturation of murine dendriticcells.
出处
《南昌大学学报（理科版）》
CAS
北大核心
2009年第3期257-260,264,共5页
Journal of Nanchang University(Natural Science)
基金
国家自然科学基金(No.30660226
20462005)
食品科学与技术国家重点实验室目标导向与自由探索项目(SKLF-MB-200806
SKLF-TS-200813)
关键词
茶叶糖蛋白
树突状细胞
细胞毒性
表型
成熟
Tea - glycoprotein
dendritic cells
cytotoxicity
phenotype
mature
