摘要
[目的]探讨双环铂对人卵巢癌细胞A2780的凋亡诱导作用并初步探索其凋亡机制。[方法]MTT法观察双环铂对A2780细胞增殖的抑制作用,荧光显微镜观察细胞形态学变化,多参数流式细胞术及TUNEL法检测细胞凋亡率,并测定caspase-3的活性变化及cas-pase抑制剂对细胞存活率的影响。[结果]双环铂可抑制A2780细胞的增殖,IC50332.9±26.3μmol/L。双环铂剂量为1×IC50、2×IC50、4×IC50时,诱导A2780细胞凋亡率分别为18.5%±5.4%、39.7%±6.0%和63.7%±3.4%,呈明显的剂量依赖性。经双环铂作用后漂浮细胞呈现典型凋亡细胞形态学改变。caspase-3活性随着细胞凋亡率增加而增加,泛caspase抑制剂z-VAD-fmk部分抑制细胞死亡。[结论]双环铂可体外诱导A2780细胞凋亡,其凋亡途径存在caspase依赖性及可能存在非caspase依赖性途径。
[Purpose] To investigate apoptosis-induced effect of dicycloplatin in human ovarian carcinoma cell line A2780, and to explore its possible mechanism. [Methods] The inhibition of dicycloplatin on A2780 cells proliferation was determined by MTT method. Morphologic change was observed by fluorescence microscopy, Annexin-V^FITC/PI staining muhiparameter flow cytometry and TUNEL assay were used to detect apoptotic ceils. The activity of caspase-3 and the effect of caspase inhibitor on cell viability were measured. [Results] Dicycloplatin inhibited cell growth with IC50 value was 332.9±26.3μmol/L. The apoptotic events produced by 1×IC50 2×IC50、4×IC50 dicycloplatin amounted to 18.5%±5.4%, 39.7%±6.0% and 63.7%±3.4%, respectively, in a dosedependent manner. Cells treated with dicyeloplatin exhibited typical morphology of apoptosis. The activity of caspase-3 increased with the increasing apoptosis, and caspase inhibitor z-VAD-fmk partly arrested cell death. [Conclusion] Dicycloplatin can induce apoptosis of A2780 in vitro. Caspase-dependent pathway involves the apoptosis and caspase-independent pathway may be involved as well.
出处
《肿瘤学杂志》
CAS
2009年第7期667-670,共4页
Journal of Chinese Oncology
