摘要
目的:探讨中药烧伤油对大鼠深Ⅱ度烫伤创面愈合的影响.方法:制备深Ⅱ度大鼠背部皮肤圆形创面,直径2.5cm,共3个,行同体对照实验.分别外用烧伤油,磺胺嘧啶锌软膏和基质软膏,1次/d.观察创面愈合情况,留取不同时相的创面组织,进行组织病理学检查,创面羟脯氨酸含量检测,ELISA法测定创面Ⅰ/Ⅲ型胶原比例和流式细胞仪测定创面真皮成纤维细胞周期.结果:外用烧伤油使创面肿胀和渗出减轻,无感染迹象及创面愈合时间缩短.病理形态学观察发现烧伤油组创面的局部组织炎症反应较轻,愈后表皮各层分化良好.羟脯氨酸和Ⅰ/Ⅲ型胶原测定显示烧伤油能促进创面胶原增生,减轻瘢痕增生.创面真皮成纤维细胞周期提示烧伤油能促进真皮成纤维细胞增殖.结论:中药烧伤油对大鼠深Ⅱ度烫伤创面具有保护和促进愈合的作用.
AIM： To investigate the effects of burn oil of Chinese medicinal herbs on wound healing in scalded rats. METHODS： Three deep-Ⅱ-degree circular skin wounds with a diameter of 2.5 cm were prepared in the back of rats and were treated respectively with burn oil of Chinese medicinal herbs, zinc sulfadiazine cream and matrix ointment 1 time per day. The healing of the wounds was observed. The wound tissue samples were harvested at different times for histopathological examination and the content of hydroxyproline, type Ⅰ/Ⅲ collagen ratio and the fibrocyte cycle of the dermal wound were detected by ELISA assay and flow cytometry. RESULTS ： The application of burn oil of Chinese medicinal herbs reduced the swelling and effusion and shortened the healing time of the wound with no wound infection. Pathological examination showed that burn oil of Chinese medicinal herbs alleviated topical inflammation. All layers of derma were well-differentiated. Effect of hydroxyproline and wound type Ⅰ/Ⅲ collagen ratio showed that burn oil of Chinese medicinal herbs promoted the collage hyperplasy of wounds and palliated scars. The observation of the fibrocyte cycle showed that burn oil of Chinese medicinal herbs promoted the dermal fibrocyte proliferation. CONCLUSION： Burn oil of Chinese medicinal herbs promotes the wound healing in scalded rats.
出处
《第四军医大学学报》
CAS
北大核心
2009年第15期1402-1405,共4页
Journal of the Fourth Military Medical University
基金
陕西中医药管理局资助项目(07-85)
