摘要
参照GenBank中收录的鹅细小病毒(GPV)B株基因序列设计并合成了扩增GPV分离株的3对引物,利用PCR技术扩增7株小鹅瘟病毒的非结构蛋白基因和结构蛋白基因。然后将其克隆到pMD18-T载体上,筛选重组质粒并进行了序列测定及分析,测序结果表明,GPV分离株非结构蛋白基因由1884个核苷酸组成,编码627个氨基酸;结构蛋白由2199个核苷酸组成,编码732个氨基酸。拼接非结构蛋白和结构蛋白的全序列,不同毒株的同源性分别为94.3%～99.2%和92.6%～98.5%。表明目前国内的鹅细小病毒的同源性比较高,但是强弱毒株也存在一定的差别。
According to the reported complete nucteotide sequence of GPV in GenBank, three pairs of primers were designed and synthesized. The structural protein gene and non-structural protein gene of sev- en strains of GPV were amplified by PCR, then the amplified fragments were cloned into pMD18-T vector and sequenced. The results demonstrated that structural protein gene and non-stueture protein gene had 1 884 hp and 2 199 bp, and included a complet^e open reading frame which encoding a protein of 627 amino acids and 732 amino acids, respectively. The result of sequence analysis showed that all the seven strains of GPV shared highly common identity compared with GPV B strain （94. 3% -99. 2% and 92. 6%- 98.5 %）, but there is also some difference between different strains.
出处
《中国兽医杂志》
CAS
北大核心
2009年第7期11-13,共3页
Chinese Journal of Veterinary Medicine
基金
广东省科技计划项目(2004B20201023)
关键词
鹅细小病毒
非结构蛋白基因
结构蛋白基因
goose parvovirus
structural protein gene
non-structural protein gene
