摘要
目的：应用免疫组织化学方法研究血浆成份在异种移植超急性排斥反应中的作用机制。方法：用离体大鼠心脏异种血浆体外灌注，建立超急性排斥反应模型，通过人血浆及灭活补体人血浆的异种器官灌注后，以荧光免疫组织化学研究天然抗体及补体对血管内皮细胞的激活反应。结果：在超急性排斥反应中，免疫球蛋白（Ｉｇ）Ｇ、ＩｇＭ和补体Ｃ３在血管内皮细胞附着，同时，有膜攻击复合物（ＭＡＣ）存在，表明ＩｇＧ、ＩｇＭ及补体Ｃ３参与超急性排斥反应时的内皮细胞激活，并形成ＭＡＣ；灭活补体虽仍见ＩｇＭ附着于内皮细胞膜，但超急性排斥反应被阻断，内皮细胞膜也未见补体Ｃ３及ＭＡＣ的附着。结论：ＩｇＧ、ＩｇＭ和补体Ｃ３在超急性排斥反应中起重要作用，用免疫组织化学方法来研究超急性排斥反应机制是可行的。
? Objective:To study the effects of plasma components during hyperacute rejection in xenotransplantation with immunofluorescent histochemistry. Methods:The model of hyperacute rejection was established by the ex vivo xenogeneic plasma perfusion of the isolated rat heart,which was antegrade perfused with human plasma and inactivated complement human plasma(56℃,3040 minutes),respectively.To analyze the location of natural antibodyImmunoglobulin(Ig)G,IgM and the C3 on the endomyocardial surface and endothelium with immunohistochemistry. Results:In the human plasma perfusion group,the natural antibodyIgG,IgM,C3 and MAC(Membrane Attact Complex)were localized on the myocardial endothelial surface.Differently,in the inactived complement plasma perfusion group,only IgG,IgM,but no C3,MAC were localized on the same location. Conclusion:Immunohistochemical analysis is an important method to study the effect of the plasma components in xenotransplantation.The IgG,IgM and complement play some important roles in the hyperacute rejection during xenotransplantation.
出处
《中国循环杂志》
CSCD
北大核心
1998年第4期248-249,共2页
Chinese Circulation Journal
