摘要
目的：探讨细胞因子（ＴＮＦα及ＩＬ－６）水平与ＨＢＶ前Ｃ区变异之间的关系。方法：应用ＥＬＩＳＡ法及３′－碱基特异性聚合酶链反应法对４３例ＨＢＶＤＮＡ阳性慢性乙型肝炎患者进行血清ＴＮＦα、ＩＬ－６含量及ＨＢＶ前Ｃ区１８９６位点突变的检测。结果：ＨＢＶ前Ｃ区突变株，检出率为４８．８％（２１／４３），突变株感染组ＴＮＦα、ＩＬ－６水平明显高于野生株感染组。结论：慢性乙型肝炎患者血清ＴＮＦα及ＩＬ－６水平与ＨＢＶ前Ｃ区基因变异有密切关系。
Objective:This paper was to study the relationship between the level of sera eytokine and gene mutation in HBV. Methods:Using ELISA and PCR techniques, we measured the quantity of sera TNFα and IL6 and detected the mutation at 1896 in HBV precore region in HBV DNA positive patients. Results:The incidence of mutation at 1896 in HBV precore region was 48.8%, the quantity of sera TNFα and IL6 in mutational group were higher than that in wild group. Conclusions:The results indicated that sera levels of TNFα and IL6 in patients with chronic hepatitis B were related to the mutation in HBV precore region, the increasing pressure of the body's immunological system might be the cause of HBV gene mutation.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
1998年第4期371-373,共3页
Journal of China Medical University
