摘要
采用优化酶法从鳙鱼肉蛋白中制备具有清除Fenton体系产生的羟自由基(.OH)活性的抗氧化肽。应用响应面分析法以酶解液对Fenton体系产生的羟自由基(.OH)的清除率为指标优化最佳酶解工艺;酶解液相对分子质量分布采用凝胶层析法测定。复合蛋白酶是水解鳙鱼肉制备具有清除羟自由基活性酶解物的适合酶,最佳酶解条件为:温度40℃、酶解时间30 min、pH=7.0、酶加量为600 U/g底物、底物∶水=1∶2;活性肽相对分子质量范围为519～1088。响应面分析法是优化酶解工艺可行的方法;具有清除羟自由基活性的肽是一些寡肽。
The purpose of this study was to optimize enzymatic hy- drolysis parameters to product peptides with scavenging activity on hydroxyl free radical of Fenton system from bighead carp（Aristich thys nobilis） protein. The optimal hydrolysis parameters were determined by the response surface methodology. The molecular weight distribution of the hydrolysates was determined by gel filtration chromatography on Sephadex G-15 column. Protamex was the optimal enzyme to prepare peptides with scavenging activity on hydroxyl free radical from bighead carp protein. The optimal enzymatic hydrolysis conditions were： temperature 40℃, time duration 30 min, pH 7, enzyme concentration 600 U/g subsubstanee, and substance/water ratio was 1 ： 23 The molecular range of active peptides was 519 to1088. Response surface methodology was the suitable method to optimize the enzymatic hydrolysis parameters and the active peptides were some oligopeptides.
出处
《食品与机械》
CSCD
北大核心
2009年第4期64-67,共4页
Food and Machinery
关键词
鳙鱼
酶解物
生物活性肽
羟自由基
响应面分析法
Bighead carp
Enzymatic hydrolysis
Bioactive peptides
Hydroxyl free radical
Response surface methodology（RSM）
