摘要
以京玉和大久保果实为试材,对果肉和果核的解剖结构、木质素含量、过氧化酶(POD)活性及其同工酶电泳进行研究分析,旨在阐明桃果核木质化与POD的关系。结果表明,桃果核细胞从花后第2周就开始有少量木质素沉积,第4周出现较多木质化细胞,第6周大部分细胞完全木质化,果肉细胞无木质素积累;这种细胞结构变化与果肉、果核的木质素含量及POD活性变化趋势基本一致;且同工酶谱系也显示果核POD表达明显强于果肉。这表明桃果核木质化与POD有密切关系,POD特定谱系决定了木质素的合成。
The experiment took ＇Okubo＇ and ＂Jingyu＂ as material , the atomy structure, lignin contents, POD activity and isozyme electrophoresis of peach mesocarp and endocarp were studied for illustrating the relationship of endocarp lignification and POD. The results showed that little lignin deposited on the endocarp cell at second week after anthesis, more lignified cell appeared at fourth week and majority of cell completely lignified at sixth week, but lignin did not accumulate in mesocarp cell. Changes of lignin content and POD activity were basically the same trend. Based on peroxidase isozyme analysis, the expression of endocarp POD was significantly stronger than mesocarp. The experiment indicated that lignification was closely related to POD and the special isodynamic enzyme determined the biosynthesis of lignin.
出处
《西北农业学报》
CAS
CSCD
北大核心
2009年第4期272-275,共4页
Acta Agriculturae Boreali-occidentalis Sinica
基金
北京市自然科学基金重点项目(6071001)
邯郸学院硕博启动基金(2006002)
关键词
桃果核
木质化
过氧化物酶
Peach fruit endocarp
Lignification
Peroxidase
