摘要
目的:建立高效液相色谱法测定祛白糖浆中丹皮酚的含量。方法:色谱柱:Hypersil BDS柱(200mm×4.6mm,5μm);流动相:甲醇-水(45:55);流速:1.0ml·min^(-1);检测波长:274nm;柱温:30℃。结果:丹皮酚在6.21～198.72μg·ml^(-1)浓度范围内线性关系良好,r=0.999 9,平均回收率为97.5%(RSD=1.45%)。结论:该方法操作简便,快捷,准确性和重复性好,可作为祛白糖浆的质量控制方法。
Objective ： To establish a method for determination of paeonol in Qubai syrup by HPLC. Method ： Hypersil BDS eolumn（200mm ×4.6mm,5μm） was used , mobile phase was methanolwater （45：55） ,the flow rate was 1.0ml · min^-1 ,the detection wavelength was 274nm and column temperature was 30℃. Result： The calibration curve was linear within the range of 6.21 - 198. 72μg · ml ^- 1 （ r = 0. 999 9 ）. The average recovery was 97.5% （ RSD = 1.45% ）. Conclusion ： The method is convenient , accurate and reproducible , which can be used in quality control of Qubai syrup.
出处
《中国药师》
CAS
2009年第8期1095-1096,共2页
China Pharmacist
