摘要
目的:观察8-甲氧沙林对体外培养黑素细胞内游离钙浓度、细胞骨架蛋白形成的影响。方法:正常人黑素细胞取自包皮环切术切取的包皮,用激光共聚焦扫描显微镜检测细胞内游离钙浓度,用罗丹明结合毒伞素对细胞骨架蛋白进行特异性染色。结果:8-甲氧沙林可使细胞内游离钙离子浓度升高,促进黑素细胞骨架蛋白合成。结论:8-甲氧沙林可能通过诱导黑素细胞内钙离子浓度升高和细胞骨架actin蛋白生成,从而影响黑素细胞的迁移。
Objective： To investigate the effects of 8-methoxypsoralen on human melanocytes [Ca2＋]i and cytoskeleton actin organization in vitro. Methods： Human melanocytes were obtained from normal foreskins. Laser confocal microscope was employed to measure [Ca2＋]i and rhodamine-conjugated phalloidin was used to visualize the cytoskeleton actin. Results： 8 -methoxypsoralen increased [Ca2＋ ]i and induced organization of actin stress fiber cytoskeleton. Conclusion： 8-methoxypsoralen might influence the migration of melanocytes by increasing the intracellular free Ca2＋ concentration and cytoskeleton actin reorganization.
出处
《浙江大学学报（医学版）》
CAS
CSCD
北大核心
2009年第4期348-351,共4页
Journal of Zhejiang University(Medical Sciences)
基金
浙江省卫生厅科研基金资助课题(2006B057)
