摘要
水稻悬浮细胞经NaCl(0～400mmol·L-1)处理后,细胞死亡率随着NaCl浓度的增大和处理时间的延长而上升。50%饱和度的外源一氧化碳(CO)溶液预处理3h可以缓解随后由200mmol·L-1NaCl引起的细胞死亡率上升,CO清除剂血红蛋白(Hb)有逆转CO的保护效应。CO在不同程度上还可以促进超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)活性的上升或相应基因的表达。
In this study, the role of carbon monoxide （CO） on reducing cell death rate of rice suspensioncultured cells was investigated under salt stess. The cell death rate increased with the increasing of NaCl concentration （0-400 mmol.L^-1） and time （3, 6, 12 and 24 h）. Pretreatment with 50% saturation of exogenous CO aqueous solution for 3 h was able to significantly alleviate the elevating cell death rate induced by further 200 mmol.L^-1 NaCl treatment. Meanwhile, the above cytoprotective effect of CO could be reversed by the addition of hemoglobin （Hb）, a scavenger of CO. Furthermore, the activities of superoxide dismutase （SOD）, catalase （CAT） and ascorbate peroxidase （APX） or their related transcripts were also induced by CO in different degrees.
出处
《植物生理学通讯》
CSCD
北大核心
2009年第7期643-646,共4页
Plant Physiology Communications
基金
江苏省环洪泽湖生态农业生物技术重点实验室基金(HZHL0807)
国家自然科学基金(30671248)
关键词
盐胁迫
水稻悬浮细胞
一氧化碳
细胞死亡
salt stress
rice suspension-cultured cells
carbon monoxide
cell death
